Copper/Zinc Superoxide Dismutase from the Cladoceran Daphnia magna: Molecular Cloning and Expression in Response to Different Acute Environmental Stressors

Kai Lyu; Xuexia Zhu; Qianqian Wang; Yafen Chen; Zhou Yang

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Copper/Zinc Superoxide Dismutase from the Cladoceran Daphnia magna: Molecular Cloning and Expression in Response to Different Acute Environmental Stressors

机译：枝角水蚤铜/锌超氧化物歧化酶：分子克隆和表达对不同的急性环境应激反应。

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The copper/zinc superoxide dismutase (Cu/Zn-SOD) is a representative antioxidant enzyme that is responsible for the conversion of superoxide to oxygen and hydrogen peroxide in aerobic organisms. Cu/Zn-SOD mRNAs have been cloned from many species and employed as useful biomarkers of oxidative stresses. In the present study, we cloned Cu/Zn-SOD cDNA from the cladoceran Daphnia magna, analyzed its catalytic properties, and investigated mRNA expression patterns after exposure to known oxidative stressors. The full-length Cu/Zn-SOD of the D. magna (Dm-Cu/Zn-SOD) sequence consisted of 703 bp nucleotides, encoding 178 amino acids, showing well-conserved domains that were required for metal binding and several common characteristics. The deduced amino acid sequence of Dm-Cu/Zn-SOD showed that it shared high identity with Daphnia pulex (88%), Alvinella pompejana (56%), and Cristaria plicata (56%). The phylogenetic analysis indicated that Dm-Cu/Zn-SOD was highly homologous to D. pulex. The variation of Dm-Cu/Zn-SOD mRNA expression was quantified by real-time PCR, and the results indicated that the expression was up-regulated after 48-h exposure to copper, un-ionized ammonia, and low dissolved oxygen. This study shows that the Dm-Cu/Zn-SOD mRNA could be successfully employed as a biomarker of oxidative stress, which is a common mode of toxicity for many other aquatic hazardous materials.

机译：铜/锌超氧化物歧化酶（Cu / Zn-SOD）是一种代表性的抗氧化剂酶，负责在好氧生物中将超氧化物转化为氧气和过氧化氢。 Cu / Zn-SOD mRNA已从许多物种中克隆出来，并被用作氧化应激的有用生物标记。在本研究中，我们从枝角水蚤（Daphnia magna）克隆了Cu / Zn-SOD cDNA，分析了其催化特性，并研究了暴露于已知氧化应激源后的mRNA表达模式。 D. magna的全长Cu / Zn-SOD（Dm-Cu / Zn-SOD）序列由703 bp核苷酸组成，编码178个氨基酸，显示出金属结合所需的保守结构域和几个共同特征。推导的Dm-Cu / Zn-SOD氨基酸序列表明，它与水蚤（88％），庞贝氏菌（56％）和皱纹克里斯蒂娜（56％）具有高度同一性。系统发育分析表明，Dm-Cu / Zn-SOD与pulex pulex高度同源。 Dm-Cu / Zn-SOD mRNA表达的变化通过实时PCR定量，结果表明在暴露于铜，未电离的氨和低溶解氧下48小时后，该表达上调。这项研究表明，Dm-Cu / Zn-SOD mRNA可以成功地用作氧化应激的生物标记，氧化应激是许多其他水生有害物质的常见毒性模式。

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《Environmental Science & Technology》 |2013年第15期|8887-8893|共7页
作者
Kai Lyu; Xuexia Zhu; Qianqian Wang; Yafen Chen; Zhou Yang;
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作者单位

Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Biological Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210023, China;

Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Biological Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210023, China;

Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Biological Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210023, China;

State Key Laboratory for Lake Science and Environment, Nanjing Institute of Geography and Limnology, The Chinese Academy of Sciences, Nanjing, China;

Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Biological Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210023, China;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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1. Molecular cloning of manganese superoxide dismutase gene in the cladoceran Daphnia magna: effects of microcystin, nitrite, and cadmium on gene expression profiles. [J] . Lyu Kai, Zhu XueXia, Chen Rui, Aquatic Toxicology . 2014,第Null期

机译：锁形水蚤（Daphnia magna）中锰超氧化物歧化酶基因的分子克隆：微囊藻毒素，亚硝酸盐和镉对基因表达谱的影响。
2. Molecular cloning, characterization of copper/zinc superoxide dismutase and expression analysis of stress-responsive genes from Eisenia fetida against dietary zinc oxide [J] . Xiong W., Bai L., Muhammad R.-U.-H., Comparative biochemistry and physiology. Toxicology & pharmacology: CBP . 2012,第2期

机译：食用埃森尼亚（Eisenia fetida）抗日粮氧化锌的分子克隆，铜/锌超氧化物歧化酶的表征和应激反应基因的表达分析
3. Molecular cloning and expression patterns of copper/zinc superoxide dismutase and manganese superoxide dismutase in Musca domestica [J] . TangT., HuangD.-W., ZhouC.-Q., Gene: An International Journal Focusing on Gene Cloning and Gene Structure and Function . 2012,第2期

机译：家蝇中铜/锌超氧化物歧化酶和锰超氧化物歧化酶的分子克隆和表达模式
4. Cloning, Expression and Characterization of a Novel Iron Superoxide Dismutase from Magnetospirillum magneticum AMB-1 [C] . Xiangqian Li, Qilei Wang, Yemin Xue International Conference on Energy, Environment and Sustainable Development . 2013

机译：磁摩托列马省磁摩托列马省钢铁二氧化铁超氧化物歧化酶的克隆，表达及表征
5. The role of dietary zinc and copper-zinc superoxide dismutase gene expression in response to oxidative stress in the lung and brain. [D] . Levy, Mark A. 2003

机译：饮食中锌和铜锌超氧化物歧化酶基因表达在响应肺和脑中氧化应激中的作用。
6. Molecular cloning of the gene (SodCc1) that encodes a cytosolic copper/zinc-superoxide dismutase from rice (Oryza sativa L.). [O] . A Sakamoto, T Okumura, H Kaminaka, 1995

机译：编码水稻（Oryza sativa L.）胞质铜/锌超氧化物歧化酶基因（SodCc1）的分子克隆。
7. Expression, purification and characterisation of human copper-zinc superoxide dismutase protein and human-escherichia coli copper-zinc superoxide dismutase chimeric protein [O] . Grixti Justine May, Farrugia Claude, Hunter Gary, 2012

机译：人铜锌超氧化物歧化酶蛋白和人大肠杆菌铜锌超氧化物歧化酶嵌合蛋白的表达，纯化和鉴定
8. Results of an Acute Toxicity to the Cladoceran (Daphnia magna) under Static Test Conditions with ZMAT Number 4054113. [R] . 2010

机译：在ZmaT编号4054113的静态测试条件下对Cladoceran（Daphnia magna）的急性毒性的结果。

Copper/Zinc Superoxide Dismutase from the Cladoceran Daphnia magna: Molecular Cloning and Expression in Response to Different Acute Environmental Stressors

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