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首页> 外文期刊>Environmental Science & Technology >Performance Characteristics of qPCR Assays Targeting Human- and Ruminant-Associated Bacteroidetes for Microbial Source Tracking across Sixteen Countries on Six Continents
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Performance Characteristics of qPCR Assays Targeting Human- and Ruminant-Associated Bacteroidetes for Microbial Source Tracking across Sixteen Countries on Six Continents

机译:针对六个洲的16个国家/地区的微生物源追踪针对人类和反刍动物相关拟杆菌的qPCR分析的性能特征

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摘要

Numerous quantitative PCR assays for microbial fecal source tracking (MST) have been developed and evaluated in recent years. Widespread application has been hindered by a lack of knowledge regarding the geographical stability and hence applicability of such methods beyond the regional level. This study assessed the performance of five previously reported quantitative PCR assays targeting human-, cattle-, or ruminant-associated Bacteroidetes populations on 280 human and animal fecal samples from 16 countries across six continents. The tested cattle-associated markers were shown to be ruminant-associated. The quantitative distributions of marker concentrations in target and nontarget samples proved to be essential for the assessment of assay performance and were used to establish a new metric for quantitative source-specificity. In general, this study demonstrates that stable target populations required for marker-based MST occur around the globe. Ruminant-associated marker concentrations were strongly correlated with total intestinal Bacteroidetes populations and with each other, indicating that the detected ruminant-associated populations seem to be part of the intestinal core microbiome of ruminants worldwide. Consequently tested ruminant-targeted assays appear to be suitable quantitative MST tools beyond the regional level while the targeted human-associated populations seem to be less prevalent and stable, suggesting potential for improvements in human-targeted methods.
机译:近年来,已经开发并评估了用于微生物粪便来源跟踪(MST)的许多定量PCR分析方法。由于缺乏有关地理稳定性的知识,因此这种方法的适用性超出了区域水平,因此阻碍了其广泛应用。这项研究评估了六种大陆上16个国家/地区的280个人类和动物粪便样本上针对人类,牛或反刍动物相关拟杆菌的五种先前报道的定量PCR分析的性能。测试的牛相关标记显示为反刍动物相关。事实证明,目标和非目标样品中标志物浓度的定量分布对于评估检测性能至关重要,并用于建立定量来源特异性的新指标。总的来说,这项研究表明,基于标记的MST所需的稳定目标人群遍布全球。反刍动物相关的标记物浓度与肠道细菌总数和彼此之间密切相关,表明检测到的反刍动物相关种群似乎是全世界反刍动物肠道核心微生物组的一部分。因此,经过测试的以反刍动物为靶标的检测方法似乎是适合区域以外的定量MST工具,而以人为靶标的相关人群似乎不那么普遍和稳定,这表明有可能改进以人为靶标的方法。

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  • 来源
    《Environmental Science & Technology》 |2013年第15期|8548-8556|共9页
  • 作者单位

    Research Group Environmental Microbiology and Molecular Ecology, Research Division Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Vienna, Austria,InterUniversity Cooperation Centre Water & Health, Vienna, Austria,Research Group Environmental Microbiology and Molecular Ecology, Institute of Chemical Engineering, Vienna University of Technology, Gumpendorfer Strasse 1a/ 166-5-2, A-1060 Vienna, Austria;

    Environment & Public Health Research Unit, School of Environment and Technology, University of Brighton, Brighton, U.K.;

    Research Group Environmental Microbiology and Molecular Ecology, Research Division Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Vienna, Austria;

    Research Group Environmental Microbiology and Molecular Ecology, Research Division Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Vienna, Austria;

    CSIRO Land and Water, Brisbane, Australia;

    Water and Environment Technology, Chalmers University of Technology, Gothenburg, Sweden;

    Department of Microbiology, University of Barcelona, Barcelona, Spain;

    Institute of Hydraulic Engineering and Water Resources Management, Vienna University of Technology, Vienna, Austria;

    Department of Biochemistry, Makerere University, Kampala, Uganda;

    University of Kentucky, Lexington, Kentucky, United States;

    ALS Water Sciences Group, Sydney, Australia;

    College of Agriculture and Environmental Sciences, Blue Nile Water Institute, Bahir Dar University;

    Seoul National University, Seoul, Korea;

    National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands;

    Sokoine University, Morogoro, Tanzania;

    Laboratorio de Aguas y Suelos, Instituto de Investigaciones para la Industrie Quimica (INIQUI), Consejo Nacional de Investigaciones Cientificas y Tecnicas and Universidad National de Salta, Salta, Argentina;

    Tribhuvan University, Kathmandu, Nepal;

    Laboratorio de Aguas y Suelos, Instituto de Investigaciones para la Industrie Quimica (INIQUI), Consejo Nacional de Investigaciones Cientificas y Tecnicas and Universidad National de Salta, Salta, Argentina;

    Bavarian Environment Agency, Munich, Germany;

    Institute for Hygiene and Applied Immunology, Water Hygiene, Medical University of Vienna, Vienna, Austria,InterUniversity Cooperation Centre Water & Health, Vienna, Austria;

    Environment & Public Health Research Unit, School of Environment and Technology, University of Brighton, Brighton, U.K.;

    Biological Institute, Department of Microbiology, Eoetvoes Lorand University, Budapest, Hungary;

    Veterinary State Laboratory, Tulcea, Romania;

    University of California-Davis, Davis, California, United States;

    Research Group Environmental Microbiology and Molecular Ecology, Research Division Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Vienna, Austria;

    Research Group Environmental Microbiology and Molecular Ecology, Research Division Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Vienna, Austria,InterUniversity Cooperation Centre Water & Health, Vienna, Austria;

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