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首页> 外文期刊>Environmental Science & Technology >Perchlorate Reduction Using Free and Encapsulated Azospira oryzae Enzymes
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Perchlorate Reduction Using Free and Encapsulated Azospira oryzae Enzymes

机译：使用游离和封装的米曲霉酶降低高氯酸盐

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摘要

Existing methods for perchlorate remediation are hampered by the common co-occurrence of nitrate, which is structurally similar and a preferred electron acceptor. In this work, the potential for perchlorate removal using cell-free bacterial enzymes as biocatalysts was investigated using crude cell lysates and soluble protein fractions of Azospira oryzae PS, as well as soluble protein fractions encapsulated in lipid and polymer vesicles. The crude lysates showed activities between 41 700 to 54400 U L~(-1) (2.49 to 3.06 U mg~(-1) total protein). Soluble protein fractions had activities of 15 400 to 29 900 U L~(-1) (170 to 1.97 U mg~(-1)) and still retained an average of 58.2% of their original activity after 23 days of storage at 4 ℃ under aerobic conditions. Perchlorate was removed by the soluble protein fraction at higher rates than nitrate. Importantly, perchlorate reduction occurred even in the presence of 500-fold excess nitrate. The soluble protein fraction retained its function after encapsulation in lipid or polymer vesicles, with activities of 13.8 to 70.7 U L~(-1), in agreement with theoretical calculations accounting for the volume limitation of the vesicles. Further, encapsulation mitigated enzyme inactivation by proteinase K. Enzyme-based technologies could prove effective at perchlorate removal from water cocontaminated with nitrate or sulfate.
机译：现有的高氯酸盐修复方法受到硝酸盐的共同共存的阻碍，硝酸盐在结构上是相似的并且是优选的电子受体。在这项工作中，研究了使用无细胞细菌酶作为生物催化剂去除高氯酸盐的可能性，方法是使用粗细细胞裂解物和米曲霉PS的可溶性蛋白部分，以及包裹在脂质和聚合物囊泡中的可溶性蛋白部分。粗裂解物显示出41 700至54400 U L〜（-1）的活性（2.49至3.06 U mg〜（-1）的总蛋白）。可溶性蛋白组分的活度为15400至29900 UL〜（-1）（170至1.97 U mg〜（-1）），在4℃下于23℃下保存后仍保留平均原始活性的58.2％。有氧条件。高氯酸盐被可溶性蛋白质部分去除的速率高于硝酸盐。重要的是，即使存在500倍过量的硝酸盐，高氯酸盐的还原反应也会发生。可溶性蛋白组分包封在脂质或聚合物囊泡中后仍保持其功能，其活性为13.8至70.7 U L〜（-1），这与理论计算相符，说明了囊泡的体积限制。此外，封装减轻了蛋白酶K引起的酶失活。基于酶的技术可以证明有效地去除了被硝酸盐或硫酸盐共污染的水中的高氯酸盐。

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  • 来源
    《Environmental Science & Technology》 |2013年第17期|9934-9941|共8页
  • 作者

    Justin M. Hutchison; Sean K. Poust; Manish Kumar; Donald M. Cropek; Irene E. MacAllister; Clint M. Arnett; Julie L. Zilles;

  • 作者单位

    Department of Civil and Environmental Engineering, University of Illinois, Urbana, Illinois 61801, United States;

    Department of Civil and Environmental Engineering, University of Illinois, Urbana, Illinois 61801, United States;

    Department of Civil and Environmental Engineering, University of Illinois, Urbana, Illinois 61801, United States Department of Chemical Engineering, Pennsylvania State University, 155 Fenkse Lab, University Park, PA 16802;

    U.S. Army Engineer Research and Development Center, Construction Engineering Research Laboratory, Champaign, Illinois 61822, United States;

    U.S. Army Engineer Research and Development Center, Construction Engineering Research Laboratory, Champaign, Illinois 61822, United States;

    U.S. Army Engineer Research and Development Center, Construction Engineering Research Laboratory, Champaign, Illinois 61822, United States;

    3230C Newmark Civil Engineering Laboratory, MC250, 205 North Mathews Avenue, Urbana, IL 61801 Department of Civil and Environmental Engineering, University of Illinois, Urbana, Illinois 61801, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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