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首页> 外文期刊>Environmental Science & Technology >Expression Pattern of Entire Cytochrome P450 Genes and Response of Defensomes in the Benzo[a]pyrene-Exposed Monogonont Rotifer Brachionus koreanus
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Expression Pattern of Entire Cytochrome P450 Genes and Response of Defensomes in the Benzo[a]pyrene-Exposed Monogonont Rotifer Brachionus koreanus

机译：苯并[a] py暴露的单核轮虫韩国轮虫中整个细胞色素P450基因的表达模式和防御小体的响应

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摘要

Cytochrome P450 (CYP) proteins are involved in the first line of detoxification mechanism against diverse polycyclic aromatic hydrocarbons (PAHs) including benzo[a]pyrene (B[a]P).In aquatic invertebrates,there is still a lack of knowledge on the CYP genes involved in the molecular response to B[a]P exposure due to limited gene information.In this study,we cloned the entire 25 CYP genes in the monogonont rotifer Brachionus koreanus with the aid of next generation sequencing (NGS) technologies and analyzed their transcript profiles with a real-time RT-PCR array to better understand B[a]P-triggered molecular response over different time courses.As a result,B[a]P exposure induced CYP2/3-involved detoxification mechanisms and defensome,including phase Ⅱ detoxification and antioxidant systems with a modulation of the chaperone heat shock protein (hsp) expression but did not change expression of other CYP clans in B.koreanus.Therefore,we found that B[a]P induced a strong detoxification mechanism to overcome detrimental effects of B[a]P associated with B[a]P-induced growth retardation as a trade-off in fitness costs.Also,this approach revealed that the entire CYP profiling can be a way of providing a better understanding on the mode of action of B[a]P in B.koreanus with respect to molecular defense metabolism.
机译：细胞色素P450（CYP）蛋白参与了针对包括苯并[a] re（B [a] P）在内的多种多环芳烃（PAHs）的解毒机理的第一阶段。在水生无脊椎动物中，仍然缺乏对这些化合物的了解。由于有限的基因信息，CYP基因参与了对B [a] P暴露的分子反应。在这项研究中，我们借助下一代测序（NGS）技术在轮虫Brachionus koreanus中克隆了全部25个CYP基因。实时RT-PCR阵列显示其转录本特征，以更好地了解B [a] P触发的不同时间过程中的分子反应。结果，B [a] P暴露诱导CYP2 / 3参与的解毒机制和防御体，通过调节伴侣热休克蛋白（hsp）表达而改变了Ⅱ型排毒和抗氧化系统，但并未改变朝鲜族CYP氏族的表达。因此，我们发现B [a] P诱导了强烈的排毒机制。克服B [a] P与B [a] P诱导的生长迟缓相关的有害影响作为健身成本的折衷方案。此外，这种方法还揭示了整个CYP分析可以提供更好的理解分子中B [a] P在朝鲜小球藻中的分子防御代谢作用模式研究。

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  • 来源
    《Environmental Science & Technology》 |2013年第23期|13804-13812|共9页
  • 作者

    Ryeo-Ok Kim; Bo-Mi Kim; Chang-Bum Jeong; David R. Nelson; Jae-Seong Lee; Jae-Sung Rhee;

  • 作者单位

    Department of Chemistry,College of Natural Sciences,Hanyang University,Seoul 133-791,South Korea;

    Department of Chemistry,College of Natural Sciences,Hanyang University,Seoul 133-791,South Korea;

    Department of Chemistry,College of Natural Sciences,Hanyang University,Seoul 133-791,South Korea;

    Department of Microbiology,Immunology and Biochemistry,University of Tennessee,Memphis,Tennessee 38163,United States;

    Department of Chemistry,College of Natural Sciences,Hanyang University,Seoul 133-791,South Korea;

    Research Institute for Natural Sciences,Hanyang University,Seoul 133-791,South Korea;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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