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首页> 外文期刊>Environmental Science & Technology >Species Differences Take Shape at Nanoparticles: Protein Corona Made of the Native Repertoire Assists Cellular Interaction
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Species Differences Take Shape at Nanoparticles: Protein Corona Made of the Native Repertoire Assists Cellular Interaction

机译：物种差异在纳米颗粒上显现：由天然库组成的蛋白质电晕有助于细胞相互作用

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摘要

Cells recognize the biomolecular corona around a nanoparticle, but the biological identity of the complex may be considerably different among various species. This study explores the importance of protein corona composition for nanoparticle recognition by coelomocytes of the earthworm Eisenia fetida using E. fetida coelomic proteins (EfCP) as a native repertoire and fetal bovine serum (FBS) as a non-native reference. We have profiled proteins forming the long-lived corona around silver nanoparticles (75 nm OECD reference materials) and compared the responses of coelomocytes to protein coronas preformed of EfCP or FBS. We find that over time silver nanoparticles can competitively acquire a biological identity native to the cells in situ even in non-native media, and significantly greater cellular accumulation of the nanoparticles was observed with corona complexes preformed of EfCP (p ＜ 0.05). An EfCP-nanoparticle mimicry made with a recombinant protein, lysenin, revealed its critical contribution in the observed cell-nanoparticle response. This confirms the determinant role of the recognizable biological identity during invertebrate in vitro testing of nanoparticles. Our finding shows a case of species-specific formation of biomolecular coronas, and this suggests that the use of representative species may need careful consideration in assessing the risks associated with nanoparticles.
机译：细胞可以识别纳米粒子周围的生物分子电晕，但是复合物的生物学特性在不同物种之间可能有很大不同。这项研究探索了蛋白质电晕组合物对于using埃塞俄比亚造粒细胞的纳米细胞识别纳米颗粒的重要性，该方法使用的是大肠杆菌Fetida coelomic蛋白（EfCP）作为天然库，而胎牛血清（FBS）作为非天然参考。我们分析了在银纳米颗粒（75 nm OECD参考材料）周围形成长寿命电晕的蛋白质，并比较了内皮细胞对EfCP或FBS形成的蛋白质电晕的反应。我们发现，随着时间的流逝，即使在非天然介质中，纳米银颗粒也可以竞争性地获得原位细胞固有的生物学特性，并且使用EfCP形成的电晕复合物观察到纳米颗粒的明显更大的细胞蓄积性（p ＜0.05）。用重组蛋白lysenin制成的EfCP-纳米颗粒模拟物显示了其在观察到的细胞-纳米颗粒反应中的关键作用。这证实了无脊椎动物纳米粒子体外测试过程中可识别的生物学特性的决定性作用。我们的发现显示了生物分子电晕物种特异性形成的情况，这表明在评估与纳米颗粒相关的风险时可能需要仔细考虑使用代表性物种。

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  • 来源
    《Environmental Science & Technology》 |2013年第24期|14367-14375|共9页
  • 作者

    Yuya Hayashi; Teodora Miclaus; Carsten Scavenius; Katarzyna Kwiatkowska; Andrzej Sobota; Peter Engelmann; Janeck J. Scott-Fordsmand; Jan J. Enghild; Duncan S. Sutherland;

  • 作者单位

    iNANO Interdisciplinary Nanoscience Center, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark,Department of Bioscience-Soil Fauna Ecology and Ecotoxicology, Aarhus University, Vejlsovej 25, 8600 Silkeborg, Denmark;

    iNANO Interdisciplinary Nanoscience Center, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark;

    iNANO Interdisciplinary Nanoscience Center, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark,Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark;

    Department of Cell Biology, Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, Poland;

    Department of Cell Biology, Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, Poland;

    Department of Immunology and Biotechnology, Clinical Center, University of Pecs, Szigeti u. 12, Pecs H-7643, Hungary;

    Department of Bioscience-Soil Fauna Ecology and Ecotoxicology, Aarhus University, Vejlsovej 25, 8600 Silkeborg, Denmark;

    iNANO Interdisciplinary Nanoscience Center, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark,Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark;

    iNANO Interdisciplinary Nanoscience Center, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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