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首页> 外文期刊>Environmental Science & Technology >Insights into Lipidomic Perturbations in Zebrafish Tissues upon Exposure to Microcystin-LR and Microcystin-RR
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Insights into Lipidomic Perturbations in Zebrafish Tissues upon Exposure to Microcystin-LR and Microcystin-RR

机译：暴露于微囊藻毒素-LR和微囊藻毒素-RR后，斑马鱼组织中的脂蛋白摄动的见解。

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摘要

This work represents the first study of its kind that was conducted to evaluate changes in lipid metabolic networks following a balneation exposure of adult zebrafish to MCLR (microcystin-leucine- arginine) and MCRR (microcystin-arginine-arginine) at a sublethal dose (10 μg L~(-1)) for a period of 30 days. Following the exposure to MCLR and MCRR, gills, liver, intestine, and brain tissues were harvested for metabolite extraction. Extracted metabolites were detected using qTOF-LC-MS (time- of-flight-liquid chromatography-mass spectrometry). Metabolites were identified using Kegg pathways. The identified metabolites are shown on lipid biochemical maps to demonstrate major perturbations in the metabolic machinery. Results showed that most of the metabolic pathways under the lipid class were affected in different tissues of zebrafish following the exposure to MCLR and MCRR (10 μg L~(-1) for 30 days). The kind and flux of metabolic perturbations varied among different tissues of the organs after the exposure to MCLR and MCRR with the tissues of gills being the most affected. Among the various lipid pathways, cholesterol synthesis was affected significantly as observed from the highest number of perturbed metabolites in that pathway. Cholesterol is responsible for synthesis of steroid hormones and bile acids, which have been recognized as endocrine signaling molecules. Disruption in the synthesis of these compounds following MCLR/MCRR exposure suggests that MCs are capable of causing endocrine disruption among aquatic organisms even under sublethal conditions. Apart from cholesterol synthesis, various other metabolic pathways belonging to the class of essential fatty acids and lipid oxidation were also observed to be perturbed following a balneation exposure of zebrafish to MCLR/MCRR.
机译：这项工作是同类研究中的第一个研究，该研究旨在评估成年斑马鱼在亚致死剂量（10致死剂量）对MCLR（微囊藻氨酸-亮氨酸-精氨酸）和MCRR（微囊藻氨酸-精氨酸-精氨酸）的平衡处理后脂质代谢网络的变化。微克L〜（-1）），持续30天。暴露于MCLR和MCRR之后，收集g，肝，肠和脑组织用于代谢物提取。使用qTOF-LC-MS（飞行时间-液相色谱-质谱法）检测提取的代谢物。使用Kegg途径鉴定代谢产物。鉴定出的代谢物显示在脂质生化图谱上，以证明代谢机制中的主要扰动。结果显示，暴露于MCLR和MCRR（10μgL〜（-1）30天）后，斑马鱼的不同组织中大多数脂类下的代谢途径均受到影响。暴露于MCLR和MCRR后，器官的不同组织之间的代谢扰动的种类和通量各不相同，with组织受到的影响最大。在各种脂质途径中，从该途径中最高的代谢产物数量来看，胆固醇的合成受到显着影响。胆固醇负责类固醇激素和胆汁酸的合成，这些激素和胆汁酸已被公认为是内分泌信号分子。这些化合物在MCLR / MCRR暴露后的合成中断表明，即使在亚致死条件下，MC仍能引起水生生物内分泌的破坏。除胆固醇合成外，在斑马鱼的平衡处理暴露于MCLR / MCRR之后，还观察到了其他多种代谢途径，这些代谢途径都属于必需脂肪酸和脂质氧化类别。

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  • 来源
    《Environmental Science & Technology》 |2013年第24期|14376-14384|共9页
  • 作者

    Shruti Pavagadhi; Siria Natera; Ute Roessner; Rajasekhar Balasubramanian;

  • 作者单位

    Singapore-Delft Water Alliance, Block E1A, #07-03 No.l Engineering Drive 2, Singapore 117576,Department of Civil and Environmental Engineering National University of Singapore, Block E1A, #07-03 No.l Engineering Drive 2, Singapore 117576;

    Metabolomics, Australia, School of Botany, The University of Melbourne, 30 Flemington Road, Victoria 3010, Australia,Australian Centre for Plant Functional Genomics, School of Botany, The University of Melbourne, 3010 Victoria, Australia;

    Metabolomics, Australia, School of Botany, The University of Melbourne, 30 Flemington Road, Victoria 3010, Australia,Australian Centre for Plant Functional Genomics, School of Botany, The University of Melbourne, 3010 Victoria, Australia;

    Singapore-Delft Water Alliance, Block E1A, #07-03 No.l Engineering Drive 2, Singapore 117576,Department of Civil and Environmental Engineering National University of Singapore, Block E1A, #07-03 No.l Engineering Drive 2, Singapore 117576;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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