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首页> 外文期刊>Environmental Science & Technology >Antibiotic Resistance Gene-Carrying Plasmid Spreads into the Plant Endophytic Bacteria using Soil Bacteria as Carriers
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Antibiotic Resistance Gene-Carrying Plasmid Spreads into the Plant Endophytic Bacteria using Soil Bacteria as Carriers

机译：使用土壤细菌作为载体，抗生素抗性基因携带质粒蔓延到植物内生细菌中

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摘要

Applications of animal manure and treated wastewater could enrich antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in the plant microbiome. However, the mechanistic studies of the transmission of ARB and ARGs from the environment to plant endophytic bacteria were few. Herein, a genetically engineered fluorescent Escherichia coli harboring a conjugative RP4 plasmid that carries three ARGs was used to trace its spread into Arabidopsis thaliana interior in a tetracycline-amended hydroponic system in the absence or presence of a simulated soil bacterial community. Confocal microscope observation demonstrated that E. coli was internalized into plant tissues and the carried RP4 plasmid was transferred into plant endophytic bacteria. More importantly, we observed that soil bacteria inhibited the internalization of E. coli but substantially promoted RP4 plasmid spread into the plant microbiome. The altered RP4-carrying bacterial community composition in the plant microbiome and the increased core-shared RP4-carrying bacteria number between plant interior and exterior in the presence of soil bacteria collectively confirmed that soil bacteria, especially Proteobacteria, might capture RP4 from E. coli and then translocate into plant microbiome, resulting in the increased RP4 plasmid spread in the plant endophytes. Overall, our findings provided important insights into the dissemination of ARB and ARGs from the environment to the plant microbiome.
机译：动物粪肥和处理废水的应用可以在植物微生物组中富集抗生素抗性细菌（ARB）和抗生素抗性基因（Args）。然而，从植物内生细菌的环境中arb和arbs的机械研究很少。在此，使用携带三个args的共轭RP4质粒的遗传工程化荧光大肠杆菌用于在不存在或存在模拟的土壤细菌群落的情况下在四环素修正的水培系统中捕获其蔓延至拟南芥内部。共聚焦显微镜观察表明，大肠杆菌被内化到植物组织中，并将携带的RP4质粒转移到植物内生细菌中。更重要的是，我们观察到土壤细菌抑制大肠杆菌的内化，但基本上促进的RP4质粒蔓延到植物微生物组中。植物微生物组的改变的RP4携带的细菌群落组成和植物内部和植物内外的核心共享的RP4携带细菌数集体证实了土壤细菌，尤其是植物体会捕获来自大肠杆菌的RP4然后易于植物微生物组，导致植物内体蔓延的RP4质粒增加。总体而言，我们的调查结果为将Arb和从环境中的传播提供给植物微生物组的重要见解。

著录项

  • 来源
    《Environmental Science & Technology》 |2021年第15期|10462-10470|共9页
  • 作者

    Han Xu; Zeyou Chen; Ruiyang Huang; Yuxiao Cui; Qiang Li; Yanhui Zhao; Xiaolong Wang; Daqing Mao; Yi Luo; Hongqiang Ren;

  • 作者单位

    College of Environmental Science and Engineering Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria Nankai University Tianjin 300071 China;

    College of Environmental Science and Engineering Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria Nankai University Tianjin 300071 China;

    College of Environmental Science and Engineering Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria Nankai University Tianjin 300071 China;

    College of Environmental Science and Engineering Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria Nankai University Tianjin 300071 China Municipal Experimental Teaching Demonstration Center for Environmental Science and Engineering Nankai University Tianjin 300071 China;

    College of Agriculture and Forestry Science Linyi University Linyi 276000 Shandong China;

    College of Environmental Science and Engineering Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria Nankai University Tianjin 300071 China;

    College of Environmental Science and Engineering Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria Nankai University Tianjin 300071 China;

    School of Medicine Nankai University Tianjin 300071 China;

    College of Environmental Science and Engineering Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria Nankai University Tianjin 300071 China State Key Laboratory of Pollution Control and Resource Reuse School of the Environment Nanjing University Nanjing 210093 China;

    State Key Laboratory of Pollution Control and Resource Reuse School of the Environment Nanjing University Nanjing 210093 China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    antibiotic resistance; plasmid; plant microbiome; horizontal gene transfer; soil bacteria;

    机译：抗生素耐药性;质粒;植物微生物组;水平基因转移;土壤细菌;

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