Using Gene Expression to Assess the Status of Fish from Anthropogenically Influenced Estuarine Wetlands

Doris E. Vidal-Dorsch; Steven M. Bay; Monica A. Mays; Darrin J. Greenstein; Diana Young; Jeffery C. Wolf; Don Pham; Alexander V. Loguinov; Chris Vulpe

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Using Gene Expression to Assess the Status of Fish from Anthropogenically Influenced Estuarine Wetlands

机译：利用基因表达评估人为影响河口湿地鱼类的状况

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The diverse mixture of contaminants frequently present in estuaries complicates their assessment by routine chemical or biological analyses. We investigated the use of gene expression to assess contaminant exposure and the condition of southern California estuarine fish. liver gene expression, plasma estradiol concentrations, and gonad histopathology were used to study biological condition in longjaw mudsuckers {Gillichthys mirabitis). Metals, legacy organochlorine pesticides, PCBs, and contaminants of emerging concern were detected in sediments and whole fish. Overall gene expression patterns were characteristic to each of four sites investigated in this study. Differentially expressed genes belonged to several functional categories including xenobiotic metabolism, detoxification, disease, and stress responses. In general, plasma estradiol concentrations were similar among fish from all areas. Some fish gonads had pathologic changes (e.g., infection, inflammation) that could indicate weakened immune systems and chronic stress. The differential expression of some genes involved in stress responses correlated with the prevalence of histologic gonad lesions. This study indicates that gene expression is a promising tool for assessing the biological condition offish exposed to environmental contaminants.

机译：河口经常存在的各种污染物混合物使通过常规化学或生物学分析进行评估变得复杂。我们调查了使用基因表达来评估污染物暴露和南加州河口鱼类状况的情况。肝基因表达，血浆雌二醇浓度和性腺组织病理学被用于研究长爪吸吮者（Gillichthys mirabitis）的生物学状况。在沉积物和整条鱼中检测到金属，传统有机氯农药，多氯联苯和新出现的污染物。总体基因表达模式是本研究中研究的四个位点的特征。差异表达的基因属于几个功能类别，包括异种生物代谢，排毒，疾病和应激反应。一般而言，所有地区鱼类的血浆雌二醇浓度相似。一些鱼的性腺具有病理变化（例如感染，炎症），可能表明免疫系统减弱和慢性应激。与应激反应有关的一些基因的差异表达与组织性腺病变的患病率相关。这项研究表明，基因表达是评估暴露于环境污染物的鱼类生物学状况的有前途的工具。

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来源
《Environmental Science & Technology》 |2012年第1期|p.69-77|共9页
作者
Doris E. Vidal-Dorsch; Steven M. Bay; Monica A. Mays; Darrin J. Greenstein; Diana Young; Jeffery C. Wolf; Don Pham; Alexander V. Loguinov; Chris Vulpe;
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作者单位

Southem California Coastal Water Research Project, Costa Mesa, California, United States;

Southem California Coastal Water Research Project, Costa Mesa, California, United States;

Southem California Coastal Water Research Project, Costa Mesa, California, United States;

Southem California Coastal Water Research Project, Costa Mesa, California, United States;

Southem California Coastal Water Research Project, Costa Mesa, California, United States;

Experimental Pathology Laboratories, Inc., Sterling, Virginia, United States;

University of California at Berkeley, Berkeley, California, United States;

University of California at Berkeley, Berkeley, California, United States;

University of California at Berkeley, Berkeley, California, United States;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
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4. Assessing the Feedback Connections of Anthropogenic Influences on Ecosystem Performance in an Estuarine Lagoon System [C] . Le Hong VU, Maria Consuelo SANTAMARIA-FERRADA, Ron JOHNSTONE Water and environment technology conference . 2019

机译：在河口泻湖系统中评估人为因素对生态系统性能的反馈联系
5. Fish distribution patterns in Pennsylvania: Anthropogenic influences and the status of rare fishes. [D] . Argent, David Gordon. 2000

机译：宾夕法尼亚州的鱼类分布模式：人为影响和稀有鱼类的状况。
6. Tumor RAS Gene Expression Levels Are Influenced by the Mutational Status of RAS Genes and Both Upstream and Downstream RAS Pathway Genes [O] . Robert M Stephens, Ming Yi, Bailey Kessing, 2017

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7. Six practical diets were formulated to contain 2.6 (the basal diet), 48.9, 95.5, 189.83, 382.4, 779.53 mg/kg vitamin C diet. After 8 weeks of feeding, growth performance, antioxidant status, and innate immunity of juvenile pufferfish were analyzed. Results showed that specific growth rate and protein efficiency ratio in fish fed diets supplemented with vitamin C were significantly higher than in fish fed the control diet. Dietary vitamin C significantly up-regulated the expression levels of growth hormone receptors and Insulin-like growth factor. Blood cell numbers and respiratory burst activity were enhanced in fish fed vitamin C diet. Serum alkaline phosphatase, glutamic-pyruvic transaminase, and glutamic-oxaloacetic transaminase activity were significantly influenced by the dietary vitamin C levels. Fish fed diets supplemented with vitamin C showed increased hepatic superoxide dismutase, glutathione peroxidase, and catalase activity, but decreased malondialdehyde activity. In addition, we found that dietary vitamin C can significantly up-regulate the expression levels of immune-related genes (HSP70, HSP90, BAFF, TNF-α and IL-6) in liver. These results indicate that dietary vitamin C can significantly influence growth performance, antioxidant status, and innate immunity of pufferfish. [O] . Cheng C-H, Liang H-Y., Guo Z-X., 2017

机译：配制了六种实用饮食，分别含有2.6（基础饮食），48.9、95.5、189.83、382.4、779.53 mg / kg维生素C饮食。喂食8周后，分析了幼崽河豚的生长性能，抗氧化剂状态和先天免疫力。结果表明，补充维生素C的鱼饲料的比生长速率和蛋白质效率比显着高于对照组的鱼。饮食中的维生素C显着上调了生长激素受体和胰岛素样生长因子的表达水平。喂鱼的维生素C饮食可提高血细胞数量和呼吸爆发活性。饮食中的维生素C水平显着影响血清碱性磷酸酶，谷氨酸-丙酮酸转氨酶和谷氨酸-草酰乙酸转氨酶的活性。补充维生素C的鱼饲料日粮中肝超氧化物歧化酶，谷胱甘肽过氧化物酶和过氧化氢酶活性增加，但丙二醛活性降低。此外，我们发现饮食中的维生素C可以显着上调肝脏中免疫相关基因（HSP70，HSP90，BAFF，TNF-α和IL-6）的表达水平。这些结果表明，饮食中的维生素C可以显着影响河豚的生长性能，抗氧化状态和先天免疫力。

Using Gene Expression to Assess the Status of Fish from Anthropogenically Influenced Estuarine Wetlands

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