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首页> 外文期刊>Environmental Science & Technology >Size-Dependent Toxicity of Nano-C_(60) Aggregates: More Sensitive Indication by Apoptosis-Related Bax Translocation in Cultured Human Cells
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Size-Dependent Toxicity of Nano-C_(60) Aggregates: More Sensitive Indication by Apoptosis-Related Bax Translocation in Cultured Human Cells

机译：纳米C_（60）聚集体的大小依赖性毒性：通过凋亡相关Bax易位在培养的人类细胞中的更敏感的指示。

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摘要

The toxicity of NPs is not well characterized in terms of their size. In particular, the size-based toxicity of fullerene (C_(60)) remains an issue because of a lack of C_(60) NPs with a well-controlled size. In this work, six fractions of the nano-C_(60) aggregates (nC_(60)) with different size distribution were prepared by a simple differential centrifugation. By using these nC_(60) fractions, we demonstrate the size-dependent inhibition of DNA polymerase and reduced-size enhanced cytotoxicity. Above all, we found that nC_(60) NPs with smaller size may have higher toxicity potency. These size-dependent effects were observed at the high exposure doses (4-6 mg/L). Interestingly, at 20-times lower and noncytotoxic doses, the size-dependent effect can be indicated by apoptosis-related fluorescent protein fused Bax translocation. Considering the toxicity of NPs is often ignored in the traditional end-point analysis for cytotoxicity when the exposure dose is low, the findings presented here will assist in the evaluation of the size-dependent cytotoxicity and dose-response relationships of toxicity mediated by nC_(60) NPs at low doses.
机译：NPs的毒性在大小方面尚未很好地表征。特别是，由于缺乏大小控制得当的C_（60）NP，富勒烯（C_（60））的基于尺寸的毒性仍然是一个问题。在这项工作中，通过简单的差速离心制备了六种不同大小分布的纳米C_（60）聚集体（nC_（60））。通过使用这些nC_（60）馏分，我们证明了DNA聚合酶的大小依赖性抑制和减小大小的增强的细胞毒性。最重要的是，我们发现较小尺寸的nC_（60）NPs可能具有较高的毒性。在高暴露剂量（4-6 mg / L）下观察到这些大小依赖性的影响。有趣的是，在低20倍且无细胞毒性的剂量下，大小依赖性效应可以通过凋亡相关的荧光蛋白融合的Bax易位来表明。考虑到当暴露剂量低时，NPs的毒性通常在传统的细胞毒性终点分析中被忽略，因此本文介绍的发现将有助于评估nC_（）介导的毒性的大小依赖性细胞毒性和剂量-反应关系。 60）低剂量的NP。

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  • 来源
    《Environmental Science & Technology》 |2012年第6期|p.3457-3464|共8页
  • 作者

    Maoyong Song; Shaopeng Yuan; Junfa Yin; Xiaoli Wang; Zihui Meng; Hailin Wang; Guibin Jiang;

  • 作者单位

    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China;

    Department of Pharmacology, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China;

    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China;

    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China;

    Department of Pharmacology, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China;

    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China;

    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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