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首页> 外文期刊>Environmental Science & Technology >Evaluating the Effects of Bioremediation on Genotoxicity of Polycyclic Aromatic Hydrocarbon-Contaminated Soil Using Genetically Engineered, Higher Eukaryotic Cell Lines
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Evaluating the Effects of Bioremediation on Genotoxicity of Polycyclic Aromatic Hydrocarbon-Contaminated Soil Using Genetically Engineered, Higher Eukaryotic Cell Lines

机译：使用基因改造的高级真核细胞系评估生物修复对多环芳烃污染土壤遗传毒性的影响

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摘要

Bioremediation is one of the commonly applied remediation strategies at sites contaminated with polycyclic aromatic hydrocarbons (PAHs). However, remediation goals are typically based on removal of the target contaminants rather than on broader measures related to health risks. We investigated changes in the tenacity and genotoxicity of PAH-contaminated soil from a former manufactured-gas plant site before and after two simulated bioremediation processes: a sequencing batch bioreactor system and a continuous-flow column system. Toxicity and genotoxicity of the residues from solvent extracts of the soil were determined by the chicken DT40 B-lymphocyte isogenic cell line and its DNA-repair-deficient mutants. Although both bioremediation processes significantly removed PAHs from the contaminated soil (bioreactor 69% removal, column 84% removal), bioreactor treatment resulted in an increase in toxidty and genotoxicity over the course of a treatment cycle, whereas long-term column treatment resulted in a decrease in toxicity and genotoxidty. However, when screening with a battery of DT40 mutants for genotoxidty profiling, we found that column treatment induced DNA damage types that were not observed in untreated soil. Toxidty and genotoxidty bioassays can supplement chemical analysis-based risk assessment for contaminated soil when evaluating the efficacy of bioremediation.
机译：生物修复是在受多环芳烃（PAHs）污染的场所普遍采用的修复策略之一。但是，补救目标通常是基于目标污染物的去除，而不是与健康风险相关的更广泛的措施。我们在两个模拟生物修复过程之前和之后调查了一个前天然气工厂现场被PAH污染的土壤的韧度和遗传毒性的变化：两个顺序的生物反应器系统和一个连续流色谱柱系统。通过鸡DT40 B淋巴细胞同基因细胞系及其DNA修复缺陷型突变体确定了土壤溶剂提取物中残留物的毒性和遗传毒性。尽管两种生物修复工艺均能从受污染的土壤中显着去除多环芳烃（生物反应器去除69％，色谱柱去除84％），但是生物反应器处理导致整个处理过程中氧化和遗传毒性的增加，而长期色谱柱处理则导致氧化和遗传毒性的增加。降低毒性和遗传氧化。但是，当用一堆DT40突变体进行基因氧化轮廓分析时，我们发现柱处理诱导的DNA损伤类型在未处理的土壤中未观察到。当评估生物修复的功效时，Toxidation和genotoxided生物测定法可以补充基于化学分析的受污染土壤风险评估。

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  • 来源
    《Environmental Science & Technology》 |2012年第8期|p.4607-4613|共7页
  • 作者

    Jing Hu Jun Nakamura; Stephen D. Richardson; Michael D. Aitken;

  • 作者单位

    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina 27599-7431, United States;

    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina 27599-7431, United States;

    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina 27599-7431, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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