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首页> 外文期刊>Environmental Science & Technology >Raman Microspectroscopy-Based Identification of Individual Fungal Spores as Potential Indicators of Indoor Contamination and Moisture-Related Building Damage
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Raman Microspectroscopy-Based Identification of Individual Fungal Spores as Potential Indicators of Indoor Contamination and Moisture-Related Building Damage

机译：基于拉曼光谱法的个体真菌孢子鉴定，可作为室内污染和与水有关的建筑物破坏的潜在指标

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摘要

We present an application of Raman microspectroscopy (RMS) for the rapid characterization and identification of individual spores from several species of microfungi. The RMS-based methodology requires minimal sample preparation and small sample volumes for analyses. Hence, it is suitable for preserving sample integrity while providing micrometer-scale spatial resolution required for the characterization of individual cells. We present the acquisition of unique Raman spectral signatures from intact fungal spores dispersed on commercially available aluminum foil substrate. The RMS-based method has been used to compile a reference library of Raman spectra from several species of microfungi typically associated with damp indoor environments. The acquired reference spectral library has subsequently been used to identify individual microfungal spores through direct comparison of the spore Raman spectra with the reference spectral signatures in the library. Moreover, the distinct peak structures of Raman spectra provide detailed insight into the overall chemical composition of spores. We anticipate potential application of this methodology in the fields of public health, forensic sciences, and environmental microbiology.
机译：我们提出了拉曼光谱（RMS）的应用，用于快速表征和鉴定来自数种微真菌的单个孢子。基于RMS的方法需要最少的样品制备和较小的样品量进行分析。因此，它适合于保持样品完整性，同时提供表征单个细胞所需的微米级空间分辨率。我们提出了从分散在市售铝箔基材上的完整真菌孢子获得独特的拉曼光谱特征的方法。基于RMS的方法已被用来编译来自几种通常与潮湿室内环境相关的微真菌物种的拉曼光谱参考库。随后，通过直接比较孢子拉曼光谱与库中的参考光谱特征，所获取的参考光谱库已用于识别单个微真菌孢子。此外，拉曼光谱的独特峰结构提供了对孢子整体化学成分的详细了解。我们预计该方法在公共卫生，法医学和环境微生物学领域的潜在应用。

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  • 来源
    《Environmental Science & Technology》 |2012年第11期|p.6088-6095|共8页
  • 作者

    Sutapa Ghosal; Janet M.Macher; Kadra Ahmed;

  • 作者单位

    Environmental Health Laboratory Branch, California Department of Public Health, 850 Marina Bay Parkway, Mailstop G365/EHLB, Richmond, California 94804, USA;

    Environmental Health Laboratory Branch, California Department of Public Health, Richmond, California 94804, United States;

    School of Public Health, University of California, Berkeley, California 94702, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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