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首页> 外文期刊>Environmental Science & Technology >Changes in Bacterial Community Structure after Exposure to Silver Nanoparticles in Natural Waters
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Changes in Bacterial Community Structure after Exposure to Silver Nanoparticles in Natural Waters

机译：暴露于天然水中的银纳米颗粒后细菌群落结构的变化

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摘要

Silver nanoparticles (AgNPs) are widely used in commercial products as antibacterial agents, but AgNPs might be hazardous to the environment and natural aquatic bacterial communities. Our recent research demonstrated that AgNPs rapidly but temporarily inhibited natural bacterioplankton production. The current study investigates the mechanism for the observed bacterial reaction to AgNPs by examining how AgNPs impact bacterial abundance, metabolic activity (5-cyano-2,3-ditolyl tetrazolium chloride (CTC+) cells), and 16S rRNA community composition. Natural bacterioplankton communities were dosed with carboxy-functionalized AgNPs at four concentrations (0.01-1 mg-Ag/L), incubated in triplicate, and monitored over 5 days. Ionic silver (AgNO_3) and Milli-Q water treatments were used as a positive and negative control, respectively. Four general AgNP exposure responses, relative to the negative control, were observed: (1) intolerant, (2) impacted but recovering, (3) tolerant, and (4) stimulated phylotypes. Relationships between cell activity indicators and bacterial phylotypes, suggested that tolerant and recovering bacteria contributed the most to the community's productivity and rare bacteria phylotypes stimulated by AgNPs did not appear to contribute much to cell activity. Overall, natural bacterial communities tolerated single, low level AgNP doses and had similar activity levels to the negative control within five days of exposure, but bacterial community composition was different from that of the control.
机译：银纳米颗粒（AgNPs）被广泛用于商业产品中作为抗菌剂，但是AgNPs可能对环境和天然水生细菌群落有害。我们最近的研究表明，AgNPs快速但暂时抑制天然浮游细菌的产生。本研究通过检查AgNPs如何影响细菌丰度，代谢活性（5-氰基-2,3-二甲苯基四唑氯化物（CTC +）细胞）和16S rRNA群落组成，研究了观察到的细菌对AgNPs的反应机制。在天然浮游生物群落中，以四种浓度（0.01-1 mg-Ag / L）的羧基官能化AgNP给药，一式三份孵育，并进行5天的监测。离子银（AgNO_3）和Milli-Q水处理分别用作阳性和阴性对照。相对于阴性对照，观察到四种一般的AgNP暴露反应：（1）不耐受，（2）受到影响但正在恢复，（3）耐受，以及（4）受激系统型。细胞活性指标与细菌系统型之间的关系表明，耐性细菌和恢复细菌对社区生产力的贡献最大，而AgNPs刺激的稀有细菌系统型似乎对细胞活性没有太大贡献。总体而言，天然细菌群落在暴露后五天内可耐受单一的低水平AgNP剂量，并具有与阴性对照相似的活性水平，但细菌群落组成与对照不同。

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  • 来源
    《Environmental Science & Technology》 |2012年第16期|p.9120-9128|共9页
  • 作者

    Pranab Das; Clayton J. Williams; Roberta R. Fulthorpe; Md Ehsanul Hoque; Chris D. Metcalfe; Marguerite A. Xenopoulos;

  • 作者单位

    Environmental and Life Sciences Graduate Program, Trent University, Peterborough, ON, Canada;

    Department of Biology, Trent University, Peterborough, ON, Canada;

    Department of Physical and Environmental Sciences, University of Toronto, ON, Canada;

    Water Quality Centre, Trent University, Peterborough, ON, Canada;

    Water Quality Centre, Trent University, Peterborough, ON, Canada;

    Department of Biology, Trent University, Life and Health Sciences Building, Block D, 2140 E. Bank Dr., Peterborough, ON, K9J 7B8, Canada;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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