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首页> 外文期刊>Environmental Science & Technology >Adsorption of Insecticidal CrylAb Protein to Humic Substances.2. Influence of Humic and Fulvic Acid Charge and Polarity Characteristics
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Adsorption of Insecticidal CrylAb Protein to Humic Substances.2. Influence of Humic and Fulvic Acid Charge and Polarity Characteristics

机译：CrylAb杀虫蛋白对腐殖质的吸附作用2。腐殖酸和黄腐酸电荷及极性特性的影响

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摘要

Assessing the fate and potential risks of transgenic Cry proteins in soils requires understanding of Cry protein adsorption to soil particles. The companion paper provided evidence that patch-controlled electrostatic attraction (PCEA) and the hydrophobic effect contributed to CrylAb protein adsorption to an apolar humic acid (HA). Here, we further assess the relative importance of these contributions by comparing CrylAb adsorption to seven humic substances varying in polarity and charge, at different solution pH and ionic strength, I. CrylAb adsorption to relatively apolar HAs at I = 50 mM exhibited rapid initial rates, was extensive, and was only partially reversible at pH 5-8, whereas adsorption to more polar fulvic acids was weak and reversible or absent at pH >6. The decrease in adsorption with increasing HS polarity at all tested pH strongly supports a large contribution from the hydrophobic effect to adsorption, particularly at I = 50 mM when PCEA was effectively screened. Using insect bioassays, we further show that CrylAb adsorbed to a selected HA retained full insecticidal activity. Our results highlight the need to consider adsorption to soil organic matter in models that assess the fate of Cry proteins in soils.
机译：评估转基因Cry蛋白在土壤中的命运和潜在风险需要了解Cry蛋白对土壤颗粒的吸附。随附的论文提供了证据表明，斑片控制的静电吸引（PCEA）和疏水作用有助于CrylAb蛋白吸附到非极性腐殖酸（HA）上。在这里，我们通过比较在不同溶液pH和离子强度I下，CrylAb吸附到极性和电荷不同的七种腐殖质物质上，进一步评估了这些贡献的相对重要性。I= 50 mM时，CrylAb吸附在相对非极性HAs上的初始速率很快，广泛，并且在pH 5-8仅部分可逆，而对极性更大的黄腐酸的吸附很弱，在pH> 6时可逆或不存在。在所有测试的pH下，随着HS极性的增加，吸附量的减少都强烈支持了疏水作用对吸附的很大贡献，尤其是当有效筛选PCEA时，I = 50 mM。使用昆虫生物测定法，我们进一步表明吸附到选定HA的CrylAb保留了全部杀虫活性。我们的结果强调了在评估土壤中Cry蛋白命运的模型中需要考虑对土壤有机质的吸附。

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  • 来源
    《Environmental Science & Technology》 |2012年第18期|p.9932-9940|共9页
  • 作者

    Jeanne E. Tomaszewski; Michael Madliger; Joel A. Pedersen; Rene P. Schwarzenbach; Michael Sander;

  • 作者单位

    Swiss Federal Institute of Technology, ETH Zurich, Institute of Biogeochemistry and Pollutant Dynamics (IBP), Zurich, Switzerland;

    Swiss Federal Institute of Technology, ETH Zurich, Institute of Biogeochemistry and Pollutant Dynamics (IBP), Zurich, Switzerland;

    Environmental Chemistry and Technology Program University of Wisconsin-Madison, Madison,Wisconsin 53706, United States Department of Soil Science, University of Wisconsin-Madison, Madison,Wisconsin 53706, United States;

    Swiss Federal Institute of Technology, ETH Zurich, Institute of Biogeochemistry and Pollutant Dynamics (IBP), Zurich, Switzerland;

    Swiss Federal Institute of Technology, ETH Zurich, Institute of Biogeochemistry and Pollutant Dynamics (IBP), Zurich, Switzerland;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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