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首页> 外文期刊>Environmental Science & Technology >ATR-FTIR Spectroscopy Study of the Influence of pH and Contact Time on the Adhesion of Shewanella putrefaciens Bacterial Cells to the Surface of Hematite
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ATR-FTIR Spectroscopy Study of the Influence of pH and Contact Time on the Adhesion of Shewanella putrefaciens Bacterial Cells to the Surface of Hematite

机译：pH和接触时间对腐烂希瓦氏菌细菌细胞在赤铁矿表面粘附的影响的ATR-FTIR光谱研究

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摘要

Attachment of live cells of Shewanella putrefa-dens strain CN-32 to the surface of hematite (α-Fe_2O_3) was studied with in situ ATR-FTIR spectroscopy at variable pH (4.5-7.7) and contact times up to 24 h. The IR spectra indicate that phosphate based functional groups on the cell wall play an important role in mediating adhesion through formation of inner-sphere coordinative bonds to hematite surface sites. The inner-sphere attachment mode of microbial P groups varies with pH, involving either a change in protonation or in coordination to hematite surface sites as pH is modified. At all pH values, spectra collected during the early stages of adhesion show intense IR bands associated with reactive P-groups, suggestive of preferential coordination of P-moieties at the hematite surface. Spectra collected after longer sorption times show distinct frequencies from cell wall protein and carboxyl groups, indicating that bacterial adhesion occurring over longer time scales is to a lesser degree associated with preferential attachment of P-based bacterial functional groups to the hematite surface. The results of this study demonstrate that pH and reaction time influence cell-mineral interactions, implying that these parameters play an important role in determining cell mobility and biofihn formation in aqueous geochemical environments.
机译：用原位ATR-FTIR光谱研究了在可变pH（4.5-7.7）和接触时间长达24小时的条件下，腐烂希瓦氏菌（Shewanella putrefa-dens）菌株CN-32的活细胞与赤铁矿（α-Fe_2O_3）表面的附着。红外光谱表明，细胞壁上的磷酸酯基官能团在通过与赤铁矿表面位点形成内球配位键来介导粘附方面起着重要作用。微生物P基团的内球附着模式随pH值而变化，随着pH值的改变，质子化或与赤铁矿表面位点的配位都发生变化。在所有pH值下，在粘附初期收集的光谱都显示出与反应性P-基团相关的强烈IR波段，这暗示着赤铁矿表面P-基团的优先配位。在更长的吸附时间后收集的光谱显示出与细胞壁蛋白质和羧基不同的频率，表明在更长的时间范围内发生的细菌粘附程度较小，与基于P的细菌官能团优先附着于赤铁矿表面有关。这项研究的结果表明，pH和反应时间会影响细胞与矿物质的相互作用，这意味着这些参数在确定含水地球化学环境中的细胞迁移率和生物膜形成中起着重要作用。

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  • 来源
    《Environmental Science & Technology》 |2012年第23期|12848-12855|共8页
  • 作者

    Evert J. Elzinga; Jen-How Huang; Jon Chorover; Ruben Kretzschmar;

  • 作者单位

    Department of Earth & Environmental Sciences, Rutgers University, Newark, New Jersey, United States;

    Institute of Biogeochemistry and Pollutant Dynamics, ETH Zurich, Zurich, Switzerland;

    Department of Soil, Water and Environmental Science, University of Arizona, Tucson, Arizona, United States;

    Institute of Biogeochemistry and Pollutant Dynamics, ETH Zurich, Zurich, Switzerland;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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