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Temperature and Humidity Influences on Inactivation Kinetics of Enteric Viruses on Surfaces

机译:温度和湿度对肠道病毒在表面上失活动力学的影响

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摘要

Norovirus (NoV) and hepatitis A virus (HAV) are pathogenic enteric viruses responsible for public health concerns worldwide. The viral transmission occurs through fecally contaminated food, water, fomites, or direct contact. However, the difficulty in cultivating these viruses makes it a challenge to characterize the resistance to various environmental stresses. In this study, we characterized the inactivation rates of murine norovirus (MNV), MS2, and HAV on either lacquer coating rubber tree wood or stainless steel under different temperature and relative humidity (RH) conditions. The viruses were analyzed at temperatures of 15 ℃, 25 ℃, 32 ℃, and 40 ℃ and at RHs of 30%, 50%, and 70% for 30 days. Overall, they survived significantly longer on wood than on steel at lower temperature (P < 0.05). The inactivation rate of MS2 and MNV increased at higher RH levels, whereas HAV survived the best at a medium RH level (50%). The effect of RH was significant only for MS2 (P < 0.05). MS2 persisted longest under ail of the environmental conditions examined. Both a linear and a nonlinear Weibull model were used to describe the viral inactivation data in this study. The data produced a better fit to the survival curves that were predicted by the Weibull model.
机译:诺如病毒(NoV)和甲型肝炎病毒(HAV)是引起全球公共卫生问题的致病性肠病毒。病毒传播是通过受粪便污染的食物,水,污染物或直接接触而发生的。然而,培养这些病毒的困难使表征对各种环境胁迫的抗性成为挑战。在这项研究中,我们表征了在不同温度和相对湿度(RH)条件下,涂漆橡胶树木材或不锈钢上的鼠诺如病毒(MNV),MS2和HAV的失活率。在15℃,25℃,32℃和40℃的温度下以及30%,50%和70%的相对湿度下分析病毒30天。总体而言,它们在较低温度下在木材上的存活时间明显长于在钢上的存活时间(P <0.05)。在较高的RH水平下,MS2和MNV的失活率增加,而在中等RH水平(50%)下,HAV存活最好。 RH的影响仅对MS2显着(P <0.05)。在所有所检查的环境条件下,MS2持续时间最长。线性和非线性Weibull模型均用于描述该研究中的病毒灭活数据。数据对Weibull模型预测的生存曲线产生了更好的拟合。

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  • 来源
    《Environmental Science & Technology》 |2012年第24期|13303-13310|共8页
  • 作者单位

    Department of Environmental Health, Graduate School of Public Health, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, South Korea,These authors contributed equally to this work;

    Department of Environmental Health, Graduate School of Public Health, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, South Korea,These authors contributed equally to this work;

    Department of Environmental Health, Graduate School of Public Health, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, South Korea;

    Department of Environmental Health, Graduate School of Public Health, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, South Korea;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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