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首页> 外文期刊>Environmental Science & Technology >Mechanistic Study of 17α-Ethinylestradiol Biodegradation by Pleurotus ostreatus: Tracking of Extracelullar and Intracelullar Degradation Mechanisms
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Mechanistic Study of 17α-Ethinylestradiol Biodegradation by Pleurotus ostreatus: Tracking of Extracelullar and Intracelullar Degradation Mechanisms

机译：平菇菌降解17α-乙炔雌二醇的机理研究：追踪胞外和鞘内降解机制

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摘要

The white rot fungus Pleurotus ostreatus is able to completely remove the synthetic hormone 17α-etbinylestradiol (EE2, 200 μg in 20 mL) from a liquid complex or mineral medium in 3 or 14 days, respectively. Its efficiency has also been documented in the removal of estrogenic activity that correlated with the EE2 degradation. A set of in vitro experiments using various cellular and enzyme fractions has been performed and the results showed that EE2 was degraded by isolated laccase (about 90% within 24 h). The degradation was also tested with concentrated extracellular liquid where degradation reached 50% mainly due to the laccase activity; however, after a supplementation with H_2O_2 and Mn~(2+), residual manganese-dependent peroxidase activities (40 times lower than Lac) raised the degradation to 100%. Moreover, the intracellular fraction and also laccase-like activity associated with fungal mycelium were found to be efficient in the degradation too. Isolated microsomal proteins appeared to also be involved in the process. The degradation was completely suppressed in the presence of cytochrome P-450 inhibitors, piperonylbutoxide and carbon monoxide, indicating a role of this monooxygenase in the degradation process. Attention was also paid to monitoring of changes in the estrogenic activity during these particular in vitro experiments when mainly degradations related to ligninolytic enzymes were found to decrease the estrogenic activity with EE2 removal proportionally. Several novel metabolites of EE2 were detected using different chromatographic method with mass spectrometric techniques (LC-MS, GC-MS) including also [~(13)C]-labeled substrates. The results document the involvement of various different simultaneous mechanisms in the EE2 degradation by P. ostreatus by both the ligninolytic system and the eukaryotic machinery of cytochromes P-450.
机译：白腐菌侧耳可以分别在3或14天内从液体复合物或矿物质培养基中完全去除合成激素17α-戊二烯雌二醇（EE2，200μg，20 mL）。在消除与EE2降解相关的雌激素活性方面也已证明了其效率。已进行了一系列使用各种细胞和酶部分的体外实验，结果表明EE2被分离的漆酶降解（24小时内约90％）。还使用浓缩的细胞外液测试了降解，其中主要由于漆酶活性，降解达到50％。然而，在补充H_2O_2和Mn〜（2+）之后，残留的锰依赖性过氧化物酶活性（比Lac低40倍）将降解提高到100％。此外，发现与真菌菌丝体相关的细胞内级分以及漆酶样活性在降解中也是有效的。分离的微粒体蛋白似乎也参与了该过程。在细胞色素P-450抑制剂，胡椒基丁醇和一氧化碳的存在下，降解被完全抑制，表明这种单加氧酶在降解过程中的作用。在这些特定的体外实验中，当发现主要与木质素分解酶有关的降解降低了雌激素活性并按比例去除EE2时，也要注意监测雌激素活性的变化。使用不同的色谱方法和质谱技术（LC-MS，GC-MS）检测到了EE2的几种新型代谢物，其中还包括[〜（13）C]标记的底物。结果证明，木质素分解系统和细胞色素P-450的真核机制都通过多种不同的同时发生机制参与了平菇对EE2的降解。

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  • 来源
    《Environmental Science & Technology》 |2012年第24期|13377-13385|共9页
  • 作者

    Zdena Kresinova; Monika Moeder; Martin Ezechias; Katerina Svobodova; Tomas Cajthaml;

  • 作者单位

    Institute of Microbiology, Academy of Sciences of the Czech Republic, v.vi., Videnska 1083, CZ-142 20 Prague 4, Czech Republic,Institute of Environmental Studies, Faculty of Science, Charles University, Benatska 2, CZ-128 01 Prague 2, Czech Republic;

    Department of Analytical Chemistry, Centre for Environmental Research Leipzig-Halle, Permoserstrasse 15, D-04318 Leipzig, Germany;

    Institute of Microbiology, Academy of Sciences of the Czech Republic, v.vi., Videnska 1083, CZ-142 20 Prague 4, Czech Republic,Institute of Environmental Studies, Faculty of Science, Charles University, Benatska 2, CZ-128 01 Prague 2, Czech Republic;

    Institute of Microbiology, Academy of Sciences of the Czech Republic, v.vi., Videnska 1083, CZ-142 20 Prague 4, Czech Republic;

    Institute of Microbiology, Academy of Sciences of the Czech Republic, v.vi., Videnska 1083, CZ-142 20 Prague 4, Czech Republic;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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