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首页> 外文期刊>Environmental Science & Technology >Tetracycline Resistance Genes Persist at Aquaculture Farms in the Absence of Selection Pressure
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Tetracycline Resistance Genes Persist at Aquaculture Farms in the Absence of Selection Pressure

机译：在没有选择压力的情况下，四环素抗性基因存在于水产养殖场

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摘要

The prophylactic and therapeutic use of tetracyclines in aquaculture has been shown to contribute to the spread of tetracycline resistance in the environment In this work, the prevalence of four different tetracyeiine-resistance genes, tetA, tetC, tetH, and tetM, in sediments from four aquaculture farms and their surroundings in the Baltic Sea was monitored by quantitative potymerase chain reaction (qPCR). The presence of three additional tetracyeiine-resistance genes (tetE, tetG, and tetWi was studied qualitatively by standard PCR, and the amount of bioavailable tetracyclines and total amounts of tetracycline and oxytetracycline in samples were also measured. None of the farms were using tetracycline at the time of the sampling and one of the farms had stopped all antibiotic use six years prior to the first sampling. Two of the farms were sampled over four successive summers and two were sampled once. Our results showed greater copy numbers of tetA, tetC, tetH, and tetM at the farms compared to pristine sites and demonstrated the presence of tetE, tetG, and tetW genes in the sediments under aquaculture farms at most sampling times. However, no resistance genes were found in samples collected 230 m from any of the farms. None of the samples contained therapeutically active concentrations of tetracyclines at any of the sampling times, suggesting that the increase in the prevalence of tetracycline resistance genes is caused by the persistence of these genes in the absence of selection pressure.
机译：四环素在水产养殖中的预防和治疗用途已证明可促进四环素抗性在环境中的传播。在这项工作中，四种不同的四环素抗性基因（tetA，tetC，tetH和tetM）在来自四个沉积物中的流行波罗的海的水产养殖场及其周围地区通过定量potymerase链反应（qPCR）进行了监测。通过标准PCR定性研究了另外三个抗四环素基因（tetE，tetG和tetWi）的存在，并测量了样品中可生物利用的四环素的含量以及四环素和土霉素的总量。采样时间，并且其中一个养殖场在第一次采样前六年就停止了所有抗生素的使用；其中两个养殖场在连续四个夏天进行了采样，两个养殖场进行了一次采样。我们的结果表明，tetA，tetC，农场中的tetH和tetM与原始位点相比，表明在大多数采样时间下水产养殖场下沉积物中都存在tetE，tetG和tetW基因，但是在任何一个采样点230 m处采集的样品中均未发现抗性基因。在任何采样时间中，没有一个样品含有治疗有效浓度的四环素，表明四环素的患病率增加在缺乏选择压力的情况下，这些基因的持久性会导致阿奇霉素抗性基因。

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  • 来源
    《Environmental Science & Technology》 |2011年第2期|p.386-391|共6页
  • 作者

    MANU IAMMINEN; ANTTI KARKMAN; ANDRES IOHMUS; WINDI INDRA MUZIASARI; HIROYUKI TAKASU; SHIGEKI WADA; SATORU SUZUKI; MARKO VIRTA;

  • 作者单位

    Department of Food and Environmental Sciences, University of Helsinki, P.O. Box 56, 00014 Helsinki, Finland;

    rnDepartment of Food and Environmental Sciences, University of Helsinki, P.O. Box 56, 00014 Helsinki, Finland;

    rnDepartment of Food and Environmental Sciences, University of Helsinki, P.O. Box 56, 00014 Helsinki, Finland;

    rnDepartment of Food and Environmental Sciences, University of Helsinki, P.O. Box 56, 00014 Helsinki, Finland;

    rnCenter for Marine Environmental Studies, Ehime University, Matsuyama, Ehime 7908577, Japan;

    rnCenter for Marine Environmental Studies, Ehime University, Matsuyama, Ehime 7908577, Japan;

    rnCenter for Marine Environmental Studies, Ehime University, Matsuyama, Ehime 7908577, Japan;

    rnDepartment of Food and Environmental Sciences, University of Helsinki, P.O. Box 56, 00014 Helsinki, Finland;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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