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首页> 外文期刊>Environmental Science & Technology >Adsorption and Aggregation Properties of Norovirus Gl and Gil Virus-like Particles Demonstrate Differing Responses to Solution Chemistry
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Adsorption and Aggregation Properties of Norovirus Gl and Gil Virus-like Particles Demonstrate Differing Responses to Solution Chemistry

机译：诺如病毒Gl和Gil病毒样颗粒的吸附和聚集性质表明对溶液化学的不同反应

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摘要

The transport properties (adsorption and aggregation behavior) of virus-like particles (VLPs) of two strains of norovirus ("Norwalk" GI.1 and "Houston" GII.4) were studied in a variety of solution chemistries. GI.1 and GII.4 VLPs were found to be stable against aggregation at pH 4.0-8.0. At pH 9.0, GI.1 VLPs rapidly disintegrated. The attachment efficiencies (a) of GI.1 and GII.4 VLPs to silica increased with increasing ionic strength in NaCI solutions at pH 8.0. The attachment efficiency of GI.1 VLPs decreased as pH was increased above the isoelectric point (pH 5.0), whereas at and below the isoelectric point, the attachment efficiency was erratic. Ca~(2+) and Mg~(2+) dramatically increased the attachment efficiencies of GI.1 and GII.4 VLPs, which may be due to specific interactions with the VLP capsids. Bicarbonate decreased attachment efficiencies for both GI.1 and GII.4 VLPs, whereas phosphate decreased the attachment efficiency of GI.1, while increasing GII.4 attachment efficiency. The observed differences in GI.1 and GII.4 VLP attachment efficiencies in response to solution chemistry may be attributed to differential responses of the unique arrangement of exposed amino acid residues on the capsid surface of each VLP strain.
机译：在多种溶液化学中研究了两种诺如病毒（“ Norwalk” GI.1和“休斯顿” GII.4）病毒样颗粒（VLP）的运输特性（吸附和聚集行为）。发现GI.1和GII.4 VLP在pH 4.0-8.0下对聚集稳定。在pH 9.0时，GI.1 VLP迅速分解。在pH 8.0的NaCl溶液中，GI.1和GII.4 VLP与二氧化硅的附着效率（a）随着离子强度的增加而增加。 GI.1 VLP的附着效率随着pH升高到等电点（pH 5.0）以上而降低，而在等电点及等电点以下，附着效率不稳定。 Ca〜（2+）和Mg〜（2+）显着提高了GI.1和GII.4 VLP的附着效率，这可能是由于与VLP衣壳的特异性相互作用所致。碳酸氢盐降低了GI.1和GII.4 VLP的附着效率，而磷酸盐降低了GI.1的附着效率，同时增加了GII.4的附着效率。 GI.1和GII.4对溶液化学反应的VLP附着效率观察到的差异可能归因于每个VLP菌株衣壳表面上暴露的氨基酸残基独特排列的不同响应。

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  • 来源
    《Environmental Science & Technology》 |2011年第2期|p.520-526|共7页
  • 作者

    ALLEGRA K. DA SILVA; OWEN V. KAVANAGH; MARY K. ESTES; MENACHEM ELIMELECH;

  • 作者单位

    Department of Chemical and Environmental Engineering, Yale University, New Haven, Connecticut 06520-8286, United States,American Association for the Advancement of Science (AAAS) Science and Technology Policy Fellow, US Agency for International Development, Washington, D.C. 20523-0016, United States;

    rnDepartment of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 77030, United States;

    rnDepartment of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 77030, United States;

    rnDepartment of Chemical and Environmental Engineering, Yale University, New Haven, Connecticut 06520-8286, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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