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首页> 外文期刊>Environmental Science & Technology >Enzyme-Immobilized Nanofiltration Membrane To Mitigate Biofouling Based on Quorum Quenching
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Enzyme-Immobilized Nanofiltration Membrane To Mitigate Biofouling Based on Quorum Quenching

机译：基于群体猝灭的酶固定化纳滤膜减轻生物污染

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摘要

Recently, enzymatic quorum quenching (in the form of a free enzyme or an immobilized form on a bead) was successfully applied to a submerged membrane bioreactor with a microfiltration membrane for wastewater treatment as a novel approach to control membrane biofouling. In this study, a quorum quenching enzyme (acylase) was directly immobilized onto a nanonltration membrane to mitigate biofouling in a nanofiltration process. In a flow cell experiment, the acylase-immobilized membrane with quorum quenching activity prohibited the formation of mushroom-shaped mature biofilm due to the reduced secretion of extracellular polymeric substances (EPS). The acylase-immobilized membrane maintained more than 90% of its initial enzyme activity for more than 20 iterative cycles of reaction and washing procedure. In the lab-scale continuous crossflow nanofiltration system operated at a constant pressure of 2 bar, the flux with the acylase-immobilized nanofiltration (NF) membrane was maintained at more than 90% of its initial flux after a 38-h operation, whereas that with the raw NF membrane decreased to 60% accompanied with severe biofouling. The quorum quenching activity of the acylase-immobilized membrane was also confirmed by visualizing the spatial distribution of cells and polysaccharides on the surface of each membrane using confocal laser scanning microscopy (CLSM) image analysis technique.
机译：最近，酶促群体猝灭（以游离酶的形式或固定在珠子上的形式）已成功应用于带有微滤膜的浸没式膜生物反应器中，用于废水处理，作为控制膜生物结垢的新方法。在这项研究中，将群体猝灭酶（酰基转移酶）直接固定在纳米滤膜上，以减轻纳滤过程中的生物污染。在流通池实验中，由于细胞外聚合物质（EPS）的分泌减少，具有群体猝灭活性的固定化酰基转移酶的膜禁止形成蘑菇状的成熟生物膜。固定化酰基转移酶的膜在超过20个反复的反应和洗涤过程迭代过程中，保持其初始酶活性的90％以上。在2 bar的恒定压力下运行的实验室规模连续错流纳滤系统中，酰基转移酶固定化纳滤（NF）膜的通量在运行38小时后保持在其初始通量的90％以上，而原始NF膜含量降低至60％，并伴有严重的生物污染。通过使用共聚焦激光扫描显微镜（CLSM）图像分析技术可视化每个膜表面上的细胞和多糖的空间分布，还可以确定酰基转移酶固定膜的群体猝灭活性。

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  • 来源
    《Environmental Science & Technology》 |2011年第4期|p.1601-1607|共7页
  • 作者

    Jae-Hyuk Kim; Dong-Chan Choi; Kyung-Min Yeon; Sang-Ryong Kim; Chung-Hak Lee;

  • 作者单位

    School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Korea;

    School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Korea;

    School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Korea;

    School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Korea;

    School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Korea;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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