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首页> 外文期刊>Environmental Science & Technology >Effects of Triclocarban, Triclosan, and Methyl Triclosan on Thyroid Hormone Action and Stress in Frog and Mammalian Culture Systems
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Effects of Triclocarban, Triclosan, and Methyl Triclosan on Thyroid Hormone Action and Stress in Frog and Mammalian Culture Systems

机译：三氯卡班，三氯生和甲基三氯生对青蛙和哺乳动物培养系统中甲状腺激素作用和应激的影响

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摘要

Triclosan (TCS) and triclocarban (TCC) are widely used broad spectrum bactericides that are common pollutants of waterways and soils. Methyl triclosan (mTCS) is the predominant bacterial TCS metabolite. Previous studies have shown that TCS disrupts thyroid hormone (TH) action; however, the effects of mTCS or TCC are not known. The present study uses the cultured frog tadpole tail fin biopsy (C-fin) assay and the TH-responsive rat pituitary GH3 cell line to assess the effects of these three chemicals (1-1000 nM) on TH signaling and cellular stress within 48 h. mRNA abundance of TH receptor y3, Rana larval keratin type I (TH-response), heat shock protein 30, and catalase (stress-response) was measured using quantitative real-time polymerase chain reaction in the C-fin assay. The TH-responsive gene transcripts encoding growth hormone, deiodinase I, and proiactin were measured in GH3 cells with the heat shock protein 70 transcript acting as a cellular stress indicator. We found alteration of stress indicators at a wide range of concentrations of TCS, mTCS, and TCC in both test systems. mTCS and TCC affected TH-responsive gene transcripts at the highest concentration in mammalian cells, whereas a modest effect included lower concentrations in the C-fin assay. In contrast, TCS did not affect TH-responsive transcripts. These results identify nontarget biological effects of these bacteriocides on amphibian and mammalian cells and suggest the TH-disrapting effects observed for TCS could be mediated through its metabolite.
机译：三氯生（TCS）和三氯卡班（TCC）是广泛使用的广谱杀菌剂，它们是水路和土壤的常见污染物。甲基三氯生（mTCS）是细菌TCS的主要代谢产物。先前的研究表明，TCS会破坏甲状腺激素（TH）的作用。但是，mTCS或TCC的作用尚不清楚。本研究使用培养的青蛙t尾鳍活检（C-fin）分析和TH反应大鼠垂体GH3细胞系来评估这三种化学物质（1-1000 nM）在48小时内对TH信号和细胞应激的影响。在C-fin分析中使用定量实时聚合酶链反应测量了TH受体y3，Rana幼虫角蛋白I型（TH响应），热休克蛋白30和过氧化氢酶（应激响应）的mRNA丰度。在GH3细胞中以热激蛋白70转录本作为细胞应激指标，测量了编码生长激素，脱碘酶I和proiactin的TH响应基因转录本。我们发现在两个测试系统中，TCS，mTCS和TCC的浓度范围很宽时，应力指标都发生了变化。 mTCS和TCC在哺乳动物细胞中以最高浓度影响TH反应基因转录本，而适度的作用包括在C-fin分析中较低浓度。相比之下，TCS不会影响TH应答转录本。这些结果确定了这些杀菌剂对两栖和哺乳动物细胞的非靶向生物学作用，并表明观察到的TCS的TH分散作用可能是通过其代谢产物介导的。

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  • 来源
    《Environmental Science & Technology》 |2011年第12期|p.5395-5402|共8页
  • 作者

    Ashley Hinther; Caleb M. Bromba; Jeremy E. Wulff; Caren C. Helbing;

  • 作者单位

    Department of Biochemistry & Microbiology, University of Victoria, P.O. Box 305S Stn CSC, Victoria, B.C., Canada, V8W 3P6;

    Department of Chemistry, University of Victoria, P.O. Box 3065 Stn CSC, Victoria, B.C., Canada, V8W 3 V6;

    Department of Chemistry, University of Victoria, P.O. Box 3065 Stn CSC, Victoria, B.C., Canada, V8W 3 V6;

    Department of Biochemistry & Microbiology, University of Victoria, P.O. Box 305S Stn CSC, Victoria, B.C., Canada, V8W 3P6;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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