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首页> 外文期刊>Environmental Science & Technology >Quantification of Electron Transfer Rates to a Solid Phase Electron Acceptor through the Stages of Biofilm Formation from Single Cells to Multicellular Communities
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Quantification of Electron Transfer Rates to a Solid Phase Electron Acceptor through the Stages of Biofilm Formation from Single Cells to Multicellular Communities

机译：通过从单细胞到多细胞社区的生物膜形成阶段量化到固相电子受体的电子传输速率。

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摘要

Microbial fuel cell (MFC) technology has enabled new insights into the mechanisms of electron transfer from dissimilatory metal reducing bacteria to a solid phase electron acceptor. Using solid electrodes as electron acceptors enables quantitative real-time measurements of electron transfer rates to these surfaces. We describe here an optically accessible, dual anode, continuous flow MFC that enables realtime microscopic imaging of anode populations as they develop from single attached cells to a mature biofilms. We used this system to characterize how differences in external resistance affect cellular electron transfer rates on a per cell basis and overall biofilm development in Shewanella oneidensis strain MR-1. When a low external resistance (100 Ω) was used, estimates of current per cell reached a maximum of 204 fA/cell (1.3 × 10~6 e~- cell~(-1) sec~(-1)), while when a higher (1 MΩ) resistance was used, only 75 fA/cell (0.4 ~ 10~6 e~- cell~(-1) sec~(-1)) was produced. The 1 MΩ anode biomass consistently developed into a mature thick biofilm with tower morphology (>50 μm thick), whereas only a thin biofilm (< 5μm thick) was observed on the 100 Ω anode. These data suggest a link between the ability of a surface to accept electrons and biofilm structure development.
机译：微生物燃料电池（MFC）技术使人们对电子从异化金属还原细菌转移到固相电子受体的机理有了新的认识。使用固体电极作为电子受体可以实时定量地测量电子向这些表面的传输速率。我们在这里描述了一种光学可访问的，双阳极连续流MFC，它能够对阳极种群从单个附着的细胞发展为成熟的生物膜进行实时显微成像。我们使用该系统表征了外抗性差异如何影响单株希瓦氏菌（Shewanella oneidensis）菌株MR-1的细胞电子转移速率和每个生物膜的整体发育。当使用低外部电阻（100Ω）时，估计每个单元的电流最大为204 fA /单元（1.3×10〜6 e〜-cell〜（-1）sec〜（-1）），而当使用更高的（1MΩ）电阻，仅产生75 fA / cell（0.4〜10〜6 e-cell〜（-1）sec〜（-1））。 1MΩ的阳极生物质始终发展成具有塔形形态的成熟厚生物膜（> 50μm厚），而在100Ω阳极上仅观察到薄的生物膜（<5μm厚）。这些数据表明表面接受电子的能力与生物膜结构发展之间的联系。

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  • 来源
    《Environmental Science & Technology》 |2010年第7期|p.2721-2727|共7页
  • 作者

    JEFFREY S. MCLEAN; GREG WANGER; YUBI A. GORBY; MARTIN WAINSTEIN; JEFF MCQUAID; SHUN ICHI ISHII; ORIANNA BRETSCHGER; HALUK BEYENAL; KENNETH H. NEALSON;

  • 作者单位

    The J. Craig Venter Institute, San Diego, CA;

    rnThe J. Craig Venter Institute, San Diego, CA;

    rnThe J. Craig Venter Institute, San Diego, CA;

    rnThe J. Craig Venter Institute, San Diego, CA;

    rnThe J. Craig Venter Institute, San Diego, CA;

    rnThe J. Craig Venter Institute, San Diego, CA;

    rnThe J. Craig Venter Institute, San Diego, CA;

    rnThe Gene and Linda Voiland, School of Chemical Engineering and Bioengineering and Center for Environmental, Sediment and Aquatic Research, Washington State University, Pullman, WA;

    rnThe J. Craig Venter Institute, San Diego, CA The Gene and Linda Voiland, School of Chemical Engineering and Bioengineering and Center for Environmental, Sediment and Aquatic Research, University of Southern California, Los Angeles, CA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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