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首页> 外文期刊>Environmental Science & Technology >Tissue-Specific In Vivo Bioconcentration of Pharmaceuticals in Rainbow Trout (Oncorhynchus mykiss) Using Space-Resolved Solid-Phase Microextraction
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Tissue-Specific In Vivo Bioconcentration of Pharmaceuticals in Rainbow Trout (Oncorhynchus mykiss) Using Space-Resolved Solid-Phase Microextraction

机译：使用空间分辨固相微萃取技术对虹鳟（Oncorhynchus mykiss）中的药物进行组织特异性体内生物浓缩

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摘要

The space-resolved solid-phase microextraction (SR-SPME) technique was employed to study the tissue-specific bioconcentration of Pharmaceuticals in live fish. The segmented design of the SPME fibers allowed for the simultaneous determination of pharmaceutical residues in fish dorsal-epaxial muscle and adipose tissue with a single SPME fiber. The miniaturized fiber endowed the technique with high spatial resolution allowing for quantification of analytes within adjacent, relatively small tissues of immature rainbow trout The pre-equilibrium sampling and kinetic calibration approach yielded efficient and accurate quantitation of Pharmaceuticals in fish tissue. The ability of the SPME method to repeatedly sample the same fish circumvents problems arising from interanimal variation, thus improving the precision of generated bioconcentration kinetic profiles. In vivo monitoring with SR-SPME was validated with in vitro liquid extraction of tissue samples using medianol. Of the nine compounds evaluated, five (atrazine, gemfibrozil, carbamazepine, ibuprofen, and fluoxetine) bioconcentrated in adipose and muscle tissue over the eight exposure days. Although the accumulation of analytes in both tissues was positively correlated, each compound partitioned with differing affinities as modified by their hydrophobicity and unique molecular structure. Water samples analyzed using the SPME technique yielded results similar to those determined by solid-phase extraction (SPE); however, SPME was more rapid and operationally much simpler. This study illustrates the application conditions for in situ SR-SPME while demonstrating the potential of these miniaturized SPME fibers for simultaneous in vivo repeated sampling of multiple tissues.
机译：采用空间分辨固相微萃取（SR-SPME）技术研究活鱼中药物的组织特异性生物浓缩。 SPME纤维的分段设计允许使用单根SPME纤维同时测定鱼背外侧肌肉和脂肪组织中的药物残留。小型化的纤维赋予了该技术很高的空间分辨率，可以对未成熟虹鳟鱼的相对较小的相邻组织内的分析物进行定量分析。平衡前的采样和动力学校准方法可对鱼组织中的药物进行高效，准确的定量。 SPME方法能够重复采样相同鱼类的能力避免了动物间变异引起的问题，从而提高了所产生的生物浓缩动力学曲线的精度。 SR-SPME的体内监测已通过使用中位数的组织样品的体外液体提取进行了验证。在评估的九种化合物中，五种（阿特拉津，吉非贝齐，卡马西平，布洛芬和氟西汀）在暴露的八天中生物浓缩在脂肪和肌肉组织中。尽管分析物在两个组织中的积累呈正相关，但每种化合物均以不同的亲和力进行分配，这些亲和力通过其疏水性和独特的分子结构进行了修饰。使用SPME技术分析的水样产生的结果类似于固相萃取（SPE）所确定的结果；但是，SPME速度更快，操作更简单。这项研究说明了原位SR-SPME的应用条件，同时展示了这些小型化SPME纤维在体内多个组织的同时体内重复采样中的潜力。

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  • 来源
    《Environmental Science & Technology》 |2010年第9期|P.3417-3422|共6页
  • 作者

    XU ZHANG; rnKEN D. OAKES; rnSHUFEN CUI; rnLESLIE BRAGG; rnMARK R. SERVOS; rnJANUSZ PAWLISZYN;

  • 作者单位

    Department of Chemistry, University of Waterloo, Ontario, N2L 3G1, Canada;

    rnDepartment of Biology, University of Waterloo, Ontario, N2L 3G1, Canada;

    rnDepartment of Chemistry, University of Waterloo, Ontario, N2L 3G1, Canada;

    rnDepartment of Biology, University of Waterloo, Ontario, N2L 3G1, Canada;

    rnDepartment of Biology, University of Waterloo, Ontario, N2L 3G1, Canada;

    rnDepartment of Chemistry, University of Waterloo, Ontario, N2L 3G1, Canada;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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