Declining Capacity of Starving Delftia acidovorans MC1 to Degrade Phenoxypropionate Herbicides Correlates with Oxidative Modification of the Initial Enzyme

SABINE LEIBELING; FRANK SCHMIDT; NICO JEHMLICH; MARTIN VON BERGEN; ROLAND H. MUELLER; HAUKE HARMS

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Declining Capacity of Starving Delftia acidovorans MC1 to Degrade Phenoxypropionate Herbicides Correlates with Oxidative Modification of the Initial Enzyme

机译：饥饿的Delftia acidovorans MC1降解苯氧丙酸类除草剂的能力下降与初始酶的氧化修饰有关。

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Bioremediation relies on the stability of enzymatic activities, particularly when bioavailable contaminant concentrations do not permit much renewal of microbial biomass. Starving Delftia acidovorans MC1 were found to lose specific degradation activity, while accumulating variants of the α-ketoglutarate-dependent dioxygenase RdpA, the enzyme initiating the degradation of (RS)-2-(2,4-dichlorophenoxy)propionate. These variants differed in their pl and originated from post-translational modification, since there is only one rdpA gene in the genome. It was tested if RdpA modification resulted from carbonylation by reactive oxygen species, known side products of dioxygenase reactions. Carbonyiated amino acids in proteins of starved cells were specifically derivatized with 2,4-dinitrophenylhydrazine. Subsequent immunolabeling of the resulting hydrazones and mass spectrometry of tryptic digests confirmed different levels of carbonylation of RdpA.

机译：生物修复依赖于酶活性的稳定性，特别是当生物可利用的污染物浓度不允许微生物生物质大量更新时。发现饥饿的Delftia acidovorans MC1失去特定的降解活性，同时积累了α-酮戊二酸依赖性双加氧酶RdpA的变体，该酶引发（RS）-2-（2,4-二氯苯氧基）丙酸酯的降解。这些变体的pl不同，并且起源于翻译后修饰，因为基因组中只有一个rdpA基因。测试了RdpA修饰是否由活性氧（双加氧酶反应的已知副产物）羰基化导致。饥饿细胞蛋白质中的羰基化氨基酸被2,4-二硝基苯肼特异性衍生。随后对所得进行的免疫标记和胰蛋白酶消化物的质谱分析证实了RdpA的羰基化水平不同。

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来源
《Environmental Science & Technology》 |2010年第10期|P.3793-3799|共7页
作者
SABINE LEIBELING; FRANK SCHMIDT; NICO JEHMLICH; MARTIN VON BERGEN; ROLAND H. MUELLER; HAUKE HARMS;
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作者单位

UFZ, Helmholtz-Centre for Environmental Research, Department Environmental Microbiology, Permoserstrasse 15, D-0431B Leipzig, Germany;

rnUFZ, Helmholtz-Centre for Environmental Research, Department Proteomics, Permoserstrasse 15, D-04318 Leipzig, Germany Interfaculty Institute for Genetics and Functional Genomics, University of Greifswald, Friedrich-Ludwig-Jahn-Strasse 15a, D-17487 Greifswald, Germany;

rnUFZ, Helmholtz-Centre for Environmental Research, Department Proteomics, Permoserstrasse 15, D-04318 Leipzig, Germany;

rnUFZ, Helmholtz-Centre for Environmental Research, Department Proteomics, Permoserstrasse 15, D-04318 Leipzig, Germany;

rnUFZ, Helmholtz-Centre for Environmental Research, Department Environmental Microbiology, Permoserstrasse 15, D-0431B Leipzig, Germany;

rnUFZ, Helmholtz-Centre for Environmental Research, Department Environmental Microbiology, Permoserstrasse 15, D-0431B Leipzig, Germany;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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1. Purification and characterisation of the enantiospecific dioxygenases from Delftia acidovorans MCI initiating the degradation of phenoxypropionate and phenoxyacetate herbicides [J] . Westendorf A, Muller RH, Babel W Acta Biotechnologica . 2003,第1期

机译：酸德尔福特菌MCI对映体特异性双加氧酶的纯化和表征，引发苯氧基丙酸酯和苯氧基乙酸除草剂的降解
2. Regulation of catabolic enzymes during long-term exposure of Delftia acidovorans MC1 to chlorophenoxy herbicides [J] . Dirk Benndorf, Ian Davidson, Wolfgang Babel Microbiology . 2004,第4期

机译：在Delftia acidia acidovorans mc1到氯苯氧基除草剂的长期暴露期间分解代谢酶的调节
3. Assimilatory detoxification of herbicides by Delftia acidovorans MC1: induction of two chlorocatechol 1,2-dioxygenases as a response to chemostress [J] . Dirk Benndorf, Wolfgang Babel Microbiology . 2002,第9期

机译：Delftia acidia acidovorans MC1的混蛋解毒：两种氯丁醇1,2-二氧化基酶的诱导作为对化学特化的反应
4. 2,4-dichlorophenoxyacetic acid-induced oxidative stress: Metabolome and membrane modifications in Umbelopsis isabellina, a herbicide degrader [O] . Przemysław Bernat, Justyna Nykiel-Szymańska, Paulina Stolarek, 2012

机译：2,4-二氯苯氧基乙酸诱导的氧化应激：伞形变种（伞形变种）的代谢组学和膜修饰
5. Purification and Characterisation of the Enantiospecific Dioxygenases from Delftia acidovorans MC1 Initiating the Degradation of Phenoxypropionate and Phenoxyacetate Herbicides [O] . A. Westendorf, R. H. Müller, W. Babel 2003

机译：来自Delftia acidia acidovorans mc1的鉴定性二氧化基酶的纯化和表征引发苯氧基丙酸酯和苯氧膦酸酯除草剂的降解

Declining Capacity of Starving Delftia acidovorans MC1 to Degrade Phenoxypropionate Herbicides Correlates with Oxidative Modification of the Initial Enzyme

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