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首页> 外文期刊>Environmental Science & Technology >Performance of PCR-Based Assays Targeting Bacteroidales Genetic Markers of Human Fecal Pollution in Sewage and Fecal Samples
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Performance of PCR-Based Assays Targeting Bacteroidales Genetic Markers of Human Fecal Pollution in Sewage and Fecal Samples

机译：针对粪便和粪便样品中人类粪便污染的细菌类遗传标记的基于PCR的检测方法的性能

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摘要

There are numerous PCR-based assays available to characterize human fecal pollution in ambient waters. Each assay employs distinct oligonucleotides and many target different genes and microorganisms leading to potential variations in assay performance. Performance comparisons utilizing feces and raw sewage samples are needed to determine which assays are best suited for expensive and time-consuming field validation, fate, transport and epidemiology studies. We report the assessment of five end-point PCR and 10 real-time quantitative PCR (qPCR) assays that target genes from presumptive Bacteroidales microorganisms reported to be associated with human feces. Each assay was tested against a reference collection of 54 primary influent sewage samples collected from different geographical locations across the United States and 174 fecal DNA extracts from 23 different animal sources. Experiments indicate that human-associated genetic markers are distributed across a broad range of human populations but show substantial differences in specificity for human feces suggesting that particular assays may be more suitable than others depending on the abundance of genetic marker required for detection and the animal sources impacting a particular watershed or beach of interest
机译：有许多基于PCR的测定可用于表征环境水域中人类粪便的污染。每种测定法均采用不同的寡核苷酸，并且许多靶标针对不同的基因和微生物，从而导致测定法性能可能发生变化。需要使用粪便和原始污水样品进行性能比较，以确定哪种测定最适合进行昂贵且耗时的现场验证，命运，运输和流行病学研究。我们报告评估了五个终点PCR和10个实时定量PCR（qPCR）分析的评估，这些分析针对的是据报道与人类粪便相关的推测的拟杆菌属微生物的基因。针对从美国不同地理位置收集的54种主要进水污水样品的参考收集物和来自23种不同动物来源的174种粪便DNA提取物进行了对照测试。实验表明，与人类相关的遗传标记物分布在广泛的人群中，但显示出对人类粪便的特异性差异很大，这表明特定的检测方法可能比其他检测方法更适合，具体取决于检测所需的遗传标记物和动物来源影响特定的分水岭或感兴趣的海滩

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  • 来源
    《Environmental Science & Technology》 |2010年第16期|p.6281-6288|共8页
  • 作者

    ORIN C. SHANKS; KAREN WHITE; CATHERINE A. KELTY; MANO SIVAGANESAN; IANET BUNNON; MARK MECKES; MANJU VARMA; RICHARD A. HAUGLAND;

  • 作者单位

    U.S. Environmental Protection Agency, Office of Research and Development, National Risk Management Research Laboratory, 26 West Martin Luther King Drive, Cincinnati,Ohio 45268;

    rnU.S. Environmental Protection Agency, Office of Research and Development, National Risk Management Research Laboratory, 26 West Martin Luther King Drive, Cincinnati,Ohio 45268;

    rnU.S. Environmental Protection Agency, Office of Research and Development, National Risk Management Research Laboratory, 26 West Martin Luther King Drive, Cincinnati,Ohio 45268;

    rnU.S. Environmental Protection Agency, Office of Research and Development, National Risk Management Research Laboratory, 26 West Martin Luther King Drive, Cincinnati,Ohio 45268;

    rnU.S. Environmental Protection Agency, Office of Research and Development, National Risk Management Research Laboratory, 26 West Martin Luther King Drive, Cincinnati,Ohio 45268;

    rnU.S. Environmental Protection Agency, Office of Research and Development, National Risk Management Research Laboratory, 26 West Martin Luther King Drive, Cincinnati,Ohio 45268;

    rnU.S. Environmental Protection Agency, Office of Research and Development, National Exposure Research Laboratory, 26 West Martin Luther King Drive,Cincinnati, Ohio 45268;

    rnU.S. Environmental Protection Agency, Office of Research and Development, National Exposure Research Laboratory, 26 West Martin Luther King Drive,Cincinnati, Ohio 45268;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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