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首页> 外文期刊>Environmental Science & Technology >Effect Of Sludge Age On The Bacterial Diversity Of Bench Scale Sequencing Batch Reactors
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Effect Of Sludge Age On The Bacterial Diversity Of Bench Scale Sequencing Batch Reactors

机译：污泥龄对台式规模分批反应器细菌多样性的影响

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Sludge age or mean cell residence time (MCRT) plays a crucial role in design and operation of wastewater treatment plants. The change in performance, for example micropollutant removal, associated with changes in MCRT is often attributed to changes in microbial diversity. We operated four identical laboratory-scale sequencing batch reactors (two test and two control) in parallel for 212 days. Sludge age was decreased gradually (from 10.4 to 2.6 days) in experimental reactors whereas it was kept constant (10.4 days) in control reactors. The reactor performance and biomass changed in a manner consistent with our understanding of the effect of sludge age on a reactors performance: the effluent quality and biomass declined with decreasing MCRT. The composition of the bacterial and ammonia-oxidizing bacterial communities in four reactors was analyzed using denaturing gradient gel electrophoresis (DGGE), and similarities in band patterns were measured using the Dice coefficient. The overall similarity between the communities in reactors run at different sludge ages was indistinguishable from the similarity in communities in reactors run at identical sludge ages. This was true for both the general bacterial communities and putative AOB communities. The number of detectable bands in DGGE profiles was also unaffected by sludge age (p ～ 0.5 in both cases). Initially, the detectable diversity of activated sludge communities in all four reactors clustered with time, regardless of their designation or sludge age; however, these clusters were only weakly supported by bootstrap analysis. However, after 135 days, a sludge age specific clustering was observed in the bacterial community but not the putative ammonia-oxidizing bacterial community. The mean self-similarity of each reactor decreased, variance increased, and the number of detectable bands in DGGE profiles decreased overtime in all reactors. The changes observed with time are consistent with ecological drift. Sludge age has a subtler and slower effect than we anticipated. However, we postulate that sludge age may be more evident in the taxa occurring below the detection limit of DGGE. New sequencing technology may help us address this hypothesis.
机译：污泥年龄或平均细胞停留时间（MCRT）在废水处理厂的设计和运行中起着至关重要的作用。与MCRT的变化相关的性能变化（例如去除微污染物）通常归因于微生物多样性的变化。我们并行操作了四个相同的实验室规模的测序批处理反应器（两个测试和两个对照），持续了212天。实验反应器中的污泥年龄逐渐降低（从10.4天到2.6天），而对照反应器中的污泥年龄保持恒定（10.4天）。反应堆性能和生物量的变化方式与我们对污泥年龄对反应堆性能的影响的理解相一致：随着MCRT的降低，出水质量和生物量下降。使用变性梯度凝胶电泳（DGGE）分析了四个反应器中细菌和氨氧化细菌群落的组成，并使用Dice系数测量了谱带模式的相似性。在不同污泥龄下运行的反应堆社区之间的总体相似性与在相同污泥龄下运行的反应堆的社区相似度是无法区分的。对于一般细菌群落和推定的AOB群落都是如此。 DGGE图谱中可检测条带的数量也不受污泥年龄的影响（两种情况下，p〜0.5）。最初，所有四个反应堆中可检测到的活性污泥群落的多样性都随时间聚集，而不论其名称或污泥年龄如何；但是，这些群集仅受到引导分析的弱支持。但是，在135天后，在细菌群落中观察到了污泥龄特定的簇，但未在假定的氨氧化细菌群落中观察到。随着时间的推移，每个反应堆中每个反应堆的平均自相似度均降低，方差增加，并且DGGE谱中可检测谱带的数量逐渐减少。随着时间的推移观察到的变化与生态漂移一致。污泥年龄比我们预期的要微妙而缓慢。但是，我们推测在DGGE的检出限以下发生的分类单元中，污泥年龄可能更明显。新的测序技术可以帮助我们解决这个假设。

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  • 来源
    《Environmental Science & Technology》 |2009年第8期|2950-2956|共7页
  • 作者

    ALPER TUNGA AKARSUBASI; OZGE EYICE; IAN MISKIN; IAN M. HEAD; THOMAS P. CURTIS;

  • 作者单位

    Molecular Biology and Genetics Department, Istanbul Technical University, 34469 Istanbul, Turkey;

    Molecular Biology and Genetics Department, Istanbul Technical University, 34469 Istanbul, Turkey;

    School of Civil Engineering and Geosciences, University of Newcastle upon Tyne, Newcastle NE3 4RP U.K.;

    School of Civil Engineering and Geosciences, University of Newcastle upon Tyne, Newcastle NE3 4RP U.K.;

    School of Civil Engineering and Geosciences, University of Newcastle upon Tyne, Newcastle NE3 4RP U.K.;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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