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首页> 外文期刊>Environmental Science & Technology >Increased Pollution-induced Bacterial Community Tolerance To Sulfadiazine In Soil Hotspots Amended With Artificial Root Exudates
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Increased Pollution-induced Bacterial Community Tolerance To Sulfadiazine In Soil Hotspots Amended With Artificial Root Exudates

机译：人工根系分泌物修正的土壤热点地区对磺胺嘧啶的污染引起的细菌群落耐受性增加

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摘要

Sulfadiazine (SDZ) residues constitute an important pollutant in soils that may increase environmental reservoirs of antibiotic resistance. Our primary aim was to compare the development of pollution-induced community tolerance (PICT) to SDZ concentration levels in bulk soil and nutrient amended soil hotspots. Agricultural soil microcosms were amended with different concentrations of SDZ with or without weekly additions of artificial root exudates corresponding to realistic rhizodepo-sition rates. Bacterial community tolerance to SDZ residues, as determined by the [~3H]leucine incorporation technique, increased progressively with elevated SDZ exposure, and was significantly increased in soil hotspots (LOEC of 1 μq kg~(-1)). An alternative PICT approach based on single-cell esterase probing by flow cytometry failed to demonstrate SDZ impacts. Bacterial growth rates ([H~3]Ieucine incorporation) were significantly reduced in both bulk soil and hotspots 24 h after amendment with environmentally relevant concentrations of SDZ, while soil respiration remained unaffected even at 100 μg SDZ g~(-1). Our study for the first time demonstrates a drastically increased PICT response of a soil bacterial community due to increased carbon substrate amendment per se. Hence, hotspot soil environments such as rhizosphere and manure-soil interfaces may comprise key sites for proliferation of bacteria that are resistant or tolerant to antibiotics.
机译：磺胺嘧啶（SDZ）残留物是土壤中的重要污染物，可能会增加环境中的抗生素耐药性。我们的主要目的是比较散装土壤和养分改良土壤热点中污染引起的社区耐受性（PICT）与SDZ浓度水平的发展。用不同浓度的SDZ修改农业土壤微观世界，并添加或不添加每周对应于实际根际定位速率的人工根系分泌物。通过[〜3H]亮氨酸掺入技术确定的细菌群落对SDZ残留的耐受性，随着SDZ暴露量的增加而逐渐增加，并且在土壤热点中显着增加（LOEC为1μqkg〜（-1））。通过流式细胞术基于单细胞酯酶探测的另一种PICT方法未能证明SDZ的影响。在与环境相关的SDZ浓度修正后24小时，散装土壤和热点中的细菌生长速率（[H〜3] Ieucine掺入）均显着降低，即使在100μgSDZ g〜（-1）下，土壤呼吸仍然不受影响。我们的研究首次表明，由于碳底物本身的增加，土壤细菌群落的PICT响应急剧增加。因此，热点土壤环境（例如根际和粪肥-土壤界面）可能包含细菌繁殖的关键部位，这些细菌对抗生素具有抗性或耐受性。

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  • 来源
    《Environmental Science & Technology》 |2009年第8期|2963-2968|共6页
  • 作者

    KRISTIAN K. BRANDT; OLE R.SJOHOLM; KRISTINE A.KROGH; BENT HALLING-SORENSEN; OLE NYBROE;

  • 作者单位

    Department of Agriculture and Ecology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark;

    Department of Agriculture and Ecology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark;

    Department of Pharmaceuticals and Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark;

    Department of Pharmaceuticals and Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark;

    Department of Agriculture and Ecology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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