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首页> 外文期刊>Environmental Science & Technology >Rapid Detection and Quantification of Aspergillus fumigatus in Environmental Air Samples Using Solid-Phase Cytometry
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Rapid Detection and Quantification of Aspergillus fumigatus in Environmental Air Samples Using Solid-Phase Cytometry

机译：固相流式细胞术快速检测和定量测定环境空气样品中的烟曲霉

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摘要

Aspergillus fumigatus is an ubiquitous fungus capable of causing severe infections such as aspergilloma, allergic bronchopulmonary aspergillosis, and invasive aspergillosis, especially in immunocompromised patients. Monitoring the number of Aspergillus fumigatus spores in the air is crucial for infection control. In the present study, a novel approach for the quantification of Aspergillus fumigatus, based on solid-phase cytometry (SPC) and immunofluorescent labeling, was developed. The sensitivity and specificity of the assay were confirmed by testing pure cultures. Paecilomyces variotii and Rhizopus stolonifer were codetected but could be excluded on the basis of morphology of the microcolonies. The SPC method has considerable advantages compared to the culture-based method, including its low detection limit (4 cells/ m~3), its speed (results are obtained within 24 h), and the straightforward microscopic identification of Aspergillus fumigatus. Additionally, comparison of results obtained with both methods demonstrated that they are equally accurate for the quantification of Aspergillus fumigatus in environmental air samples.
机译：烟曲霉是一种普遍存在的真菌，​​能够引起严重的感染，例如曲霉菌瘤，过敏性支气管肺曲霉菌病和侵袭性曲霉菌病，尤其是在免疫功能低下的患者中。监测空气中的烟曲霉孢子数量对于控制感染至关重要。在本研究中，开发了一种基于固相细胞计数（SPC）和免疫荧光标记定量烟曲霉的新方法。通过检测纯培养物证实了该测定法的敏感性和特异性。杂草拟青霉（Paecilomyces variotii）和茎根霉（Rhizopus stolonifer）被编码，但根据微菌落的形态可以排除。与基于培养的方法相比，SPC方法具有显着的优势，包括检测限低（4个细胞/ m〜3），速度快（结果可在24小时内获得）以及对烟曲霉的直接显微镜鉴定。此外，通过两种方法获得的结果的比较表明，它们对环境空气样品中的烟曲霉定量分析同样准确。

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  • 来源
    《Environmental Science & Technology》 |2009年第9期|3233-3239|共7页
  • 作者

    LIES M. E. VANHEE; HANS J. NELIS; TOM COENYE;

  • 作者单位

    Laboratory of Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium;

    Laboratory of Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium;

    Laboratory of Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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