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首页> 外文期刊>Environmental Science & Technology >Communication of Radiation-Induced Signals in Vivo between DNA Repair Deficient and Proficient Medaka (Oryzias latipes)
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Communication of Radiation-Induced Signals in Vivo between DNA Repair Deficient and Proficient Medaka (Oryzias latipes)

机译：DNA修复缺陷和熟练的Medaka（Oryzias latipes）之间体内辐射诱导信号的通讯

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Radiation-induced bystander effects are established consequences of exposure to ionizing radiation. The operation of this mechanism has been seen in vitro and also between fish, mammals, and plants in vivo where stress signals from treated organisms induce responses in neighbors. In vitro research shows that DNA repair deficient cells produce more toxic bystander responses. To test this in vivo two strains of Japanese medaka were tested. One is a mutant, repair deficient strain (ric2) and the other, the wildtype repair proficient strain (CAB). Irradiated fish swam with unirradiated partners in a strain mix and match protocol. The data suggest that medaka produce signals, when exposed to radiation, that induce unirradiated fish of the same strain swimming with them to produce an altered response to that seen in bystanders to sham irradiated fish. More apoptosis was seen in bystanders to repair deficient fish. When the strains are mixed, the bystanders of either strain respond like the donor strain. Measurements of Bcl-2 and cmyc proteins in the explants confirmed these observations. A possible role for p53 was also identified in that the use of reporters with mutant p53 demonstrated that CAB signals killed all the reporter cells by apoptosis. Use of a similar but p53 wild-type cell line had no such effect. The data add to the body of knowledge showing that bystander signals operate at hierarchical levels of organization greater than the individual and may therefore have relevance in radioecology and (eco)systems biology.
机译：辐射引起的旁观者效应是暴露于电离辐射中产生的后果。已经在体外以及在鱼，哺乳动物和植物之间体内观察到了该机制的作用，其中来自处理过的生物的应激信号在邻居中诱导应答。体外研究表明，DNA修复缺陷细胞会产生更多毒性旁观者反应。为了在体内进行测试，测试了两种日本花aka菌株。一种是突变型修复缺陷菌株（ric2），另一种是野生型修复缺陷菌株（CAB）。受辐照的鱼与未辐照的伴侣在菌株混合搭配方案中游动。数据表明，被暴露于辐射时会产生信号，从而诱导未受照射的同一品系的鱼类与它们一起游泳，从而产生与旁观者观察到的对受照射的鱼的反应不同的反应。旁观者发现更多的细胞凋亡可以修复缺乏的鱼。混合菌株后，任一菌株的旁观者都会像供体菌株一样做出反应。外植体中Bcl-2和cmyc蛋白的测定证实了这些观察结果。还确定了p53的可能作用，因为将报告基因与突变体p53一起使用证明CAB信号通过凋亡杀死了所有报告细胞。使用类似但p53野生型细胞系没有这种效果。数据增加了知识体系，表明旁观者的信号在组织中的层次结构要比个人更大，因此可能与放射生态学和（生态）系统生物学有关。

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  • 来源
    《Environmental Science & Technology》 |2009年第9期|3335-3342|共8页
  • 作者

    C. MOTHERSILL; R. W. SMITH; T. G. HINTON; K. AIZAWA; C. B. SEYMOUR;

  • 作者单位

    McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada;

    McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada;

    Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, South Carolina 29802, Athens, Georgia;

    Pharmaceuticals and Medical Devices Agency, Shin-Kasumigaseki Building, 3-3-2 Kasumigaseki, Chiyoda-ku, Tokyo 100-0013, Japan;

    McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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