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首页> 外文期刊>Environmental Science & Technology >Influence of Heterogeneous Ammonium Availability on Bacterial Community Structure and the Expression of Nitrogen Fixa- tion and Ammonium Transporter Genes during in Situ Bioremediation of Uranium-Contaminated Groundwater
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Influence of Heterogeneous Ammonium Availability on Bacterial Community Structure and the Expression of Nitrogen Fixa- tion and Ammonium Transporter Genes during in Situ Bioremediation of Uranium-Contaminated Groundwater

机译：铵盐污染地下水原位生物修复过程中异质铵有效性对细菌群落结构及固氮和转运蛋白基因表达的影响

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摘要

The influence of ammonium availability on bacterial community structure and the physiological status of Geobacter species during in situ bioremediation of uranium-contaminated groundwater was evaluated. Ammonium concentrations varied by 2 orders of magnitude (<4 to 400 μM) across the study site. Analysis of 16S rRNA sequences suggested that ammonium may have been one factor influencing the community composition prior to acetate amendment with Rhodoferax species predominating over Geobacter species with higher ammonium and Dechloromonas species dominating at the site with lowest ammonium. However, once acetate was added and dissimilatory metal reduction was stimulated, Geobacter species became the predominant organisms at all locations. Rates of U(VI) reduction appeared to be more related to acetate concentrations rather than ammonium levels. In situ mRNA transcript abundance of the nitrogen fixation gene, nifD, and the ammonium transporter gene, amtB, in Geobacter species indicated that ammonium was the primary source of nitrogen during uranium reduction. The abundance of amtB was inversely correlated to ammonium levels, whereas nifD transcript levels were similar across all sites examined. These results suggest that nifD and amtB expression are closely regulated in response to ammonium availability to ensure an adequate supply of nitrogen while conserving cell resources. Thus, quantifying nifD and amtB transcript expression appears tornbe a useful approach for monitoring the nitrogen-related physiological status of subsurface Geobacter species. This study also emphasizes the need for more detailed analysis of geochemical and physiological interactions at the field scale in order to adequately model subsurface microbial processes during bioremediation.
机译：评价了在铀污染地下水的原位生物修复过程中，铵可利用性对细菌群落结构和地球细菌物种的生理状态的影响。整个研究地点的铵浓度变化了2个数量级（<4至400μM）。对16S rRNA序列的分析表明，在醋酸盐修饰之前，红景天属菌种占主导地位，而铵盐含量最高的地杆菌属和脱氯菌属物种占主导地位，铵盐可能是乙酸修饰前影响群落组成的因素。但是，一旦添加乙酸盐并刺激异化金属还原，在所有位置，土杆菌种成为主要生物。 U（VI）还原速率似乎与乙酸盐浓度而不是铵盐含量更相关。地细菌物种中固氮基因nifD和铵转运蛋白amtB的原位mRNA转录丰度表明，铵是铀还原过程中氮的主要来源。 amtB的丰度与铵水平成反比，而nifD转录本水平在所有检查部位均相似。这些结果表明，响应于铵的可用性，nifD和amtB的表达受到严格调节，以确保充足的氮供应，同时节省细胞资源。因此，量化nifD和amtB转录表达看来是监测地下地下细菌物种与氮有关的生理状态的有用方法。这项研究还强调，需要在田间规模上更详细地分析地球化学和生理学相互作用，以便在生物修复过程中充分模拟地下微生物的过程。

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  • 来源
    《Environmental Science & Technology》 |2009年第12期|4386-4392|共7页
  • 作者

    PAULA J. MOUSER; A. LUCIE NGUESSAN; HILA ELIFANTZ; DAWN E. HOLMES; KENNETH H. WILLIAMS; MICHAEL J. WILKINS; PHILIP E. LONG; DEREK R. LOVLEY;

  • 作者单位

    Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003 Sanborn, Head & Associates, 95 High Street, Portland, ME 04101;

    Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003;

    Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003;

    Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003;

    Lawrence Berkeley National Laboratory, Berkeley, California 94720;

    Department of Environmental Science, Policy and Management, University of California, Berkeley, California 94720;

    Pacific Northwest National Laboratory, Richland, Washington 99352;

    Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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