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首页> 外文期刊>Environmental Science & Technology >Evaluation of SBRC-Gastric and SBRC-lntestinal Methods for the Prediction of In Vivo Relative Lead Bioavailability in Contaminated Soils
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Evaluation of SBRC-Gastric and SBRC-lntestinal Methods for the Prediction of In Vivo Relative Lead Bioavailability in Contaminated Soils

机译:SBRC胃法和SBRC肠法在受污染土壤中体内相对铅生物利用度预测中的评估

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摘要

In this study, lead (Pb) bioaccessibility in contaminated soils was assessed using an in vitro method (SBRC) encompassing gastric (SBRC-G) and intestinal (SBRC-I) phases. Initially, bioaccessibility studies were performed with a Pb reference material (Pb acetate, 1-10 mg L~(-1)) in order to determine the influence of pH on Pb solubility. In the gastric phase (pH 1.5), Pb solubility was 100% (100 ± 2.9%, n - 16) irrespective of the Pb concentration added, however, when the pH of the intestinal phase was increased to near neutral, Pb solubility decreased to 14.3 ± 7.2%. In contaminated soils, Pb bioaccessibility varied from 35.7 to 64.1 % and 1.2 to 2.7% for SBRC-G and SBRC-I phases, respectively. When relative bioaccessibility (Rel-SBRC-I) was calculated by adjusting the dissolution of Pb from contaminated soils by the solubility of Pb acetate at pH 6.5 (intestinal phase pH), Rel-SBRC-I values ranged from 11.7-26.1%. A stepwise regression model based on Pearson correlation factors was used to determine the suitability of in vitro assays for predicting in vivo (swine assay) relative Pb bioavailability. Rel-SBRC-I provided the best estimate of in vivo relative Pb bioavailability for soils used in this study (in vivo relative Pb bioavailability [%] = Rel-SBRC-I [pH 6.5%] × 0.58 + 1.98, r~2 = 0.53). The versatility of Rel-SBRC-I was demonstrated by accurately predicting relative Pb bioavailability from other reported in vivo studies.
机译:在这项研究中,使用涵盖胃(SBRC-G)和肠道(SBRC-1)阶段的体外方法(SBRC)评估了污染土壤中铅(Pb)的生物可及性。最初,为了确定pH对Pb溶解度的影响,使用Pb参考物质(乙酸Pb,1-10 mg L〜(-1))进行了生物可及性研究。在胃相(pH 1.5)中,无论添加的Pb浓度如何,Pb的溶解度均为100%(100±2.9%,n-16),但是,当肠相的pH值增加至接近中性时,Pb的溶解度降低至14.3±7.2%。在受污染的土壤中,SBRC-G和SBRC-I相的铅生物利用率分别为35.7%至64.1%和1.2%至2.7%。当通过在pH 6.5(肠道相pH)下乙酸铅的溶解度通过调节Pb从污染土壤中的溶解度来计算相对生物利用度(Rel-SBRC-1)时,Rel-SBRC-1值的范围为11.7-26.1%。使用基于Pearson相关因子的逐步回归模型来确定体外测定用于预测体内(猪测定)相对Pb生物利用度的适用性。 Rel-SBRC-I提供了本研究中所用土壤的体内相对Pb生物利用度的最佳估算值(体内相对Pb生物利用度[%] = Rel-SBRC-1 [pH 6.5%]×0.58 + 1.98,r〜2 = 0.53)。 Rel-SBRC-1的多功能性是通过从其他已报道的体内研究中准确预测相对Pb生物利用度来证明的。

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  • 来源
    《Environmental Science & Technology》 |2009年第12期|4503-4509|共7页
  • 作者单位

    Centre for Environmental Risk Assessment and Remediation, Division of Information Technology, Engineering and the Environment, University of South Australia, Mawson Lakes Campus, Mawson Lakes, SA 5095, Australia Cooperative Research Centre for Contamination Assessment and Remediation of the Environment, Mawson Lakes, SA 5095, Australia;

    Centre for Environmental Risk Assessment and Remediation, Division of Information Technology, Engineering and the Environment, University of South Australia, Mawson Lakes Campus, Mawson Lakes, SA 5095, Australia Cooperative Research Centre for Contamination Assessment and Remediation of the Environment, Mawson Lakes, SA 5095, Australia;

    Centre for Environmental Risk Assessment and Remediation, Division of Information Technology, Engineering and the Environment, University of South Australia, Mawson Lakes Campus, Mawson Lakes, SA 5095, Australia Cooperative Research Centre for Contamination Assessment and Remediation of the Environment, Mawson Lakes, SA 5095, Australia;

    Centre for Environmental Risk Assessment and Remediation, Division of Information Technology, Engineering and the Environment, University of South Australia, Mawson Lakes Campus, Mawson Lakes, SA 5095, Australia Cooperative Research Centre for Contamination Assessment and Remediation of the Environment, Mawson Lakes, SA 5095, Australia;

    Department of Soil Science and Soil Ecology, Geographical Institute, Ruhr- University Bochum, 44780, Germany;

    Centre for Environmental Risk Assessment and Remediation, Division of Information Technology, Engineering and the Environment, University of South Australia, Mawson Lakes Campus, Mawson Lakes, SA 5095, Australia;

    Institute for Medical and Veterinary Science, Frome Road, SA 5000, Australia;

    Institute for Medical and Veterinary Science, Frome Road, SA 5000, Australia;

    Sansom Institute, School of Pharmacy and Medical Sciences, Division of Health Science, University of South Australia, City East Campus, SA 5001, Australia;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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