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首页> 外文期刊>Environmental Science & Technology >A Measurement Method for Atmospheric Ammonia and Primary Amines Based on Aqueous Sampling, OPA Derivatization and HPLC Analysis
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A Measurement Method for Atmospheric Ammonia and Primary Amines Based on Aqueous Sampling, OPA Derivatization and HPLC Analysis

机译：基于水采样，OPA衍生化和HPLC分析的大气氨和伯胺的测量方法

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摘要

A method of precolumn derivatization HPLC with fluorescence detection has been developed for the measurement of ammonia, primary methylamine, ethylamine, propylamine, and butylamine in the atmosphere. Air samples were collected by two continuously wetted glass frit/coil samplers, one directly from ambient air for the sum of gaseous and aerosol species, and the other after an acid-coated annular denuder for the aerosol species. The collection efficiency for all analvtes was found to be ≥99% at a sampling gas flow rate of 2 L min~(-1) and a scrubbing water flow rate of 0.24 mL min~(-1). The collected ammonia and primary amines were derivatized with o-ph-thalaldehyde (OPA) and n-acetyl-cysteine (NAC) reagents in an in-line derivatization coil to form highly fluorescent sulfonatoisoindole derivatives. Detailed kinetic study showed that derivatization reactions were fast, but the derivatives were not very stable. Derivatization conditions, such as reagent concentrations, derivatization medium pH, and derivatization time, were optimized to achieve maximum derivative yields for all the analytes. The derivatives were separated on a C-18 reverse-phase column using a gradient elution and detected by a fluorescence detector at an excitation wavelength of 330 nm and an emission wavelength of 471 nm. The respective lower detection limits for ammonia and for the four primary amines were 24 parts per trillion by volume (pptv) and ≤3 pptv, with a sample time resolution of about 1 min and a sampling/ analysis time of 20 min per cycle. The analytical methodology has been applied in the field measurements; results from two case studies are presented.
机译：已开发出一种具有荧光检测功能的柱前衍生HPLC方法，用于测量大气中的氨，伯甲胺，乙胺，丙胺和丁胺。空气样本是由两个连续湿润的玻璃粉/卷采样器收集的，一个直接从环境空气中获取气态和气溶胶物质的总和，另一个在经过酸包覆的环形剥蚀仪后获取。在2 L min〜（-1）的采样气体流量和0.24 mL min〜（-1）的洗涤水流量下，所有对虾的收集效率均≥99％。在串联衍生线圈中，将所收集的氨和伯胺分别用邻苯二甲醛（OPA）和正乙酰半胱氨酸（NAC）试剂衍生化，以形成高荧光性磺基异吲哚衍生物。详细的动力学研究表明，衍生化反应很快，但衍生物不是很稳定。优化了衍生化条件，例如试剂浓度，衍生化介质pH值和衍生化时间，以实现所有分析物的最大衍生产率。使用梯度洗脱在C-18反相柱上分离衍生物，并通过荧光检测器在330nm的激发波长和471nm的发射波长下进行检测。氨和四种伯胺的各自下限分别为24万亿分之体积（pptv）和≤3pptv，采样时间分辨率约为1分钟，每个循环的采样/分析时间为20分钟。分析方法已应用于现场测量；介绍了两个案例研究的结果。

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  • 来源
    《Environmental Science & Technology》 |2009年第15期|5851-5856|共6页
  • 作者

    GU HUANG; JIAN HOU; XIANLIANG ZHOU;

  • 作者单位

    Department of Environmental Health and Toxicology, School of Public Health, State University of New York at Albany, Albany, New York;

    Department of Environmental Health and Toxicology, School of Public Health, State University of New York at Albany, Albany, New York;

    Department of Environmental Health and Toxicology, School of Public Health, State University of New York at Albany, Albany, New YorkWadsworth Center, New York State Department of Health, Albany, New York, 12201;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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