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首页> 外文期刊>Environmental Science & Technology >In Vivo Studies to Elucidate the Role of Extracellular Polymeric Substances from Azotobacter in Immobilization of Heavy Metals
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In Vivo Studies to Elucidate the Role of Extracellular Polymeric Substances from Azotobacter in Immobilization of Heavy Metals

机译：体内研究阐明固氮细菌的细胞外聚合物在重金属固定中的作用

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摘要

The role of extracellular polymeric substances (EPS) produced by the heavy metal-resistant strain of Azotobacter spp. in restricting the uptake of cadmium (Cd) and chromium (Cr) by wheat plants cultivated in soils contaminated with the respective heavy metals has been demonstrated. A heavy metal-resistant strain of Azotobacter spp. was isolated and identified. Minimum inhibitory concentrations (MIC) of Cd~(2+) and CrO_4~(2-) were determined to be 20 and 10 mg L~(-1), respectively. Under in vitro conditions, the EPS produced by the strain could bind 15.17 ± 0.58 mg g~(-1) of Cd~(2+) and 21.9 ± 0.08 mg g~(-1) of CrO_4~(2-). Fourier transform infrared spectra of the EPS revealed the presence of functional groups like carboxyl (-COOH) and hydroxyl (-OH), primarily involved in metal ion binding. Under pot culture experiments, the isolated strain of Azotobacter was added to the metal-contaminated soils in the form of free cells and immobilized cells. The total Azotobacter count and plant metal concentrations under different treatments showed a negative coefficient between the Azotobacter population and plant Cd (-0.496) and Cr (-0.455). Thus it could be inferred that Azotobacter spp. is involved in metal ion complexation eitherthrough EPS orthrough cell wall lipopolysaccharides (LPS).
机译：耐重氮菌属的重金属菌株产生的细胞外聚合物质（EPS）的作用。已经证明在限制被各种重金属污染的土壤中种植的小麦植物可以限制镉（Cd）和铬（Cr）的吸收。耐重氮菌属的一种重金属菌株。被隔离并确定。 Cd〜（2+）和CrO_4〜（2-）的最低抑菌浓度（MIC）分别为20和10 mg L〜（-1）。在体外条件下，菌株产生的EPS可以结合15.17±0.58 mg g〜（-1）的Cd〜（2+）和21.9±0.08 mg g〜（-1）的CrO_4〜（2-）。 EPS的傅立叶变换红外光谱表明，主要存在于金属离子结合中的官能团如羧基（-COOH）和羟基（-OH）的存在。在盆栽实验中，将分离的固氮菌菌株以游离细胞和固定细胞的形式添加到金属污染的土壤中。在不同处理下，总固氮菌计数和植物金属浓度在固氮菌种群与植物Cd（-0.496）和Cr（-0.455）之间显示负系数。因此可以推断出固氮菌属。通过EPS或通过细胞壁脂多糖（LPS）参与金属离子络合。

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  • 来源
    《Environmental Science & Technology》 |2009年第15期|5884-5889|共6页
  • 作者

    PRACHI M. JOSHI; ASHA A. JUWARKAR;

  • 作者单位

    Environmental Biotechnology Division, National Environmental Engineering Research Institute (NEERI), Nehru Marg, Nagpur-440020, India;

    Environmental Biotechnology Division, National Environmental Engineering Research Institute (NEERI), Nehru Marg, Nagpur-440020, India;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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