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首页> 外文期刊>Environmental Science & Technology >Combining HPLC-GCXGC, GCXGC/ ToF-MS, and Selected Ecotoxicity Assays for Detailed Monitoring of Petroleum Hydrocarbon Degradation in Soil and Leaching Water
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Combining HPLC-GCXGC, GCXGC/ ToF-MS, and Selected Ecotoxicity Assays for Detailed Monitoring of Petroleum Hydrocarbon Degradation in Soil and Leaching Water

机译：结合HPLC-GCXGC，GCXGC / ToF-MS和选定的生态毒性测定法，以详细监测土壤和淋洗水中石油烃的降解

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摘要

HPLC-GCXGC/FID (high-performance liquid chromatography followed by comprehensive two-dimensional gas chromatography with flame-ionization detection) and GCXGC/ToF-MS (comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry) were used to study the biodegradation of petroleum hydrocarbons in soil microcosms during 20 weeks. Two soils were studied: one spiked with fresh diesel and one field sample containing weathered diesel-like oil. Nutrient amended and unamended samples were included. Total petroleum hydrocarbon (TPH) levels in spiked soil decreased from 15 000 to 7500 mg/kg d.m. and from 12 000 to 4000 mg/kg d.m. in the field soil. Linear alkanes and aromatic hydrocarbons were better biodegradable (>60% degraded) than iso-alkanes; cycloalkanes were least degradable (<40%). Aromatic hydrocarbons up to three rings showed better degradability than n-alkanes. GCXGC/ToF-MS analysis of leaching water showed that initially various oxygenated hydrocarbons were produced. Compound peaks seemed to move up and rightward in the GCXGC chromatograms, indicating that more polar and heavier compounds were formed as biodegradation proceeded. Nutrient amendment can increase TPH removal rates, but had adverse effects on ecotoxicity and leaching potential in our experiment This was explained by observed shifts in the soil microbial community. Ecotoxicity assays showed that residual TPH still inhibited cress (Lepidium sativum) seed germination, but the leaching water was no longertoxic toward luminescent bacteria (Vibrio fischeri).
机译：使用HPLC-GCXGC / FID（高效液相色谱，然后进行带有火焰电离检测的全面二维气相色谱）和GCXGC / ToF-MS（具有飞行时间质谱的全面二维气相色谱）进行分析研究了20周内土壤微观世界中石油碳氢化合物的生物降解。研究了两种土壤：一种土壤掺有新鲜柴油，一种土壤样品含有风化的类柴油。营养成分修正和未经修正的样本均包括在内。加标土壤中的总石油烃（TPH）水平从15000降至7500 mg / kgd.m。和12000至4000 mg / kg d.m.在田间土壤中。直链烷烃和芳烃比异烷烃具有更好的生物降解性（降解率> 60％）；环烷烃的降解性最低（<40％）。最多三个环的芳烃显示出比正构烷烃更好的降解性。浸出水的GCXGC / ToF-MS分析表明，最初会生成各种氧化烃。在GCXGC色谱图中，化合物峰似乎在向上和向右移动，表明随着生物降解的进行，形成了更多极性和更重的化合物。营养改良剂可以提高TPH去除率，但在我们的实验中对生态毒性和淋溶潜能产生不利影响。这可以通过观察到的土壤微生物群落的变化来解释。生态毒性试验表明，残留的TPH仍能抑制水芹（Lepidium sativum）种子发芽，但浸出水对发光细菌（Vibrio fischeri）不再具有毒性。

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  • 来源
    《Environmental Science & Technology》 |2009年第20期|7651-7657|共7页
  • 作者

    DEBIN MAO; RICHARD LOOKMAN; HENDRIK VAN DE WEGHE; REINHILDE WELTENS; GUIDO VANERMEN; NICOLE DE BRUCKER; LUDO DIELS;

  • 作者单位

    Flemish Institute for Technological Research (VITO), Boeretang 200, B-2400 Mol, Belgium Department of Biology, University of Antwerp, Universiteitsplein 1, B-2610 Wilrijk, Belgium;

    Flemish Institute for Technological Research (VITO), Boeretang 200, B-2400 Mol, Belgium;

    Flemish Institute for Technological Research (VITO), Boeretang 200, B-2400 Mol, Belgium;

    Flemish Institute for Technological Research (VITO), Boeretang 200, B-2400 Mol, Belgium;

    Flemish Institute for Technological Research (VITO), Boeretang 200, B-2400 Mol, Belgium;

    Flemish Institute for Technological Research (VITO), Boeretang 200, B-2400 Mol, Belgium;

    Flemish Institute for Technological Research (VITO), Boeretang 200, B-2400 Mol, Belgium Department of Biology, University of Antwerp, Universiteitsplein 1, B-2610 Wilrijk, Belgium;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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