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首页> 外文期刊>Environmental Science & Technology >Global Transcriptomic Response of Pseudomonas aeruginosa to Chlorhexidine Diacetate
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Global Transcriptomic Response of Pseudomonas aeruginosa to Chlorhexidine Diacetate

机译：铜绿假单胞菌对氯己定双乙酸盐的总体转录组学反应

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摘要

Pseudomonas aeruginosa is implicated in nosocomial infections and chronic respiratory infections in cystic fibrosis patients. Chlorhexidine diacetate (CHX) is a biguanide disinfectant used for bacterial control in the hospital and agricultural and domestic environments. A better understanding of the mechanism of action of CHX and the resulting response elicited by P. aeruginosa to CHX will facilitate its effective utilization for P. aeruginosa control in these environments. This study presents, for the first time, the transcriptomic response of P. aeruginosa to 0.008 mM CHX after 10 and 60 min. Our results reveal that after both treatment times, membrane transport, oxidative phosphorylation, and electron transport genes were down-regulated. After 10 min, DNA repair was downregulated and the oprH gene that blocks the self-promoted uptake of antimicrobials was upregulated. After 60 min, outer membrane protein, flagellum, pilus, oxidative phosphorylation, and electron transport genes were downregulated. The mexC and mexD genes of the MexCD-OprJ multidrug efflux pump were significantly upregulated after both treatment times. The results of this study improve our understanding of the mode of action of CHX on P. aeruginosa and provide insights into the mechanism of action of other biguanide disinfectants which can be used for the development of more efficient disinfectants.
机译：铜绿假单胞菌与囊性纤维化患者的医院感染和慢性呼吸道感染有关。双乙酸氯己定（CHX）是一种双胍类消毒剂，用于医院，农业和家庭环境中的细菌控制。更好地了解CHX的作用机理以及铜绿假单胞菌对CHX引起的反应，将有助于其在这些环境中有效地用于铜绿假单胞菌的控制。这项研究首次提出铜绿假单胞菌在10和60分钟后对0.008 mM CHX的转录组反应。我们的结果表明，经过两个处理时间后，膜转运，氧化磷酸化和电子转运基因均被下调。 10分钟后，DNA修复被下调，而阻止抗菌素自我促进摄取的oprH基因也被上调。 60分钟后，外膜蛋白，鞭毛，菌毛，氧化磷酸化和电子转运基因被下调。在两个治疗时间后，MexCD-OprJ多药外排泵的mexC和mexD基因显着上调。这项研究的结果增进了我们对CHX对铜绿假单胞菌的作用方式的了解，并为其他双胍类消毒剂的作用机理提供了见解，这些消毒剂可用于开发更有效的消毒剂。

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  • 来源
    《Environmental Science & Technology》 |2009年第21期|8406-8415|共10页
  • 作者

    CHANTAL W. NDE; HYEUNG-JIN JANG; FRESHTEH TOGHROL; WILLIAM E. BENTIEY;

  • 作者单位

    Center for Biosystems Research University of Maryland Biotechnology Institute, College Park, Maryland 20742;

    College of Oriental Medicine, Kyung Hee University, Seoul, Korea;

    Microarray Research Laboratory, Biological and Economic Analysis Division, Office of Pesticide Programs, U.S. Environmental Protection Agency, Fort Meade, Maryland 20755;

    Center for Biosystems Research University of Maryland Biotechnology Institute, College Park, Maryland 20742;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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