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首页> 外文期刊>Environmental Science & Technology >Experimental Studies of the Influence of Grain Size, Oxygen Availability and Organic Carbon Availability on Bioclogging in Porous Media
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Experimental Studies of the Influence of Grain Size, Oxygen Availability and Organic Carbon Availability on Bioclogging in Porous Media

机译：多孔介质中颗粒大小，氧气利用率和有机碳利用率对生物阻塞影响的实验研究

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Changes in the hydraulic properties of porous material due to bioclogging have been observed in many laboratory simulations and field studies. Because such changes in hydraulic properties influence the movement of fluids and contaminants, microbial ecology data are required for improved transport modeling. Here we investigate the effects of environmental variables previously shown to influence bioclogging, specifically oxygen availability, sediment grain size, and organic carbon (nutrient) concentration on the hydraulic properties of simulated subsurface environments. Our study provides evidence of a different clogging mechanism for aerobic and anaerobic microbial communities under high organic carbon concentrations (400 mg L~(-1)). This work also suggests that the clogging mechanism operating in anaerobic microbial communities is more sensitive to carbon availability than that in the aerobic microbial communities. We found that grain size does have an effect on clogging, but it appears that there is a threshold carbon concentration, and therefore biomass, below which these effects are insignificant Differences between the microbial communities that developed under different oxygenation conditions were detected using 16s rRNA analysis.
机译：在许多实验室模拟和现场研究中，已经观察到由于生物堵塞引起的多孔材料水硬性的变化。由于水力特性的这种变化会影响流体和污染物的运动，因此需要微生物生态学数据来改善运输模型。在这里，我们研究了先前显示的影响生物阻塞的环境变量的影响，特别是氧气的可利用性，沉积物粒度和有机碳（营养物）的浓度，对模拟地下环境的水力特性的影响。我们的研究提供了在高有机碳浓度（400 mg L〜（-1））下好氧和厌氧微生物群落的不同堵塞机理的证据。这项工作还表明，与好氧微生物群落相比，厌氧微生物群落中的堵塞机制对碳的利用更为敏感。我们发现晶粒尺寸确实对堵塞有影响，但似乎存在阈值碳浓度，因此存在生物量，低于该阈值这些影响不明显，使用16s rRNA分析检测到了在不同氧化条件下形成的微生物群落之间的差异。 。

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