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首页> 外文期刊>Environmental Science & Technology >Selective Enzyme-Linked Immunosorbent Assay for Triclosan. Application to Wastewater Treatment Plant Effluents
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Selective Enzyme-Linked Immunosorbent Assay for Triclosan. Application to Wastewater Treatment Plant Effluents

机译：三氯生的选择性酶联免疫吸附测定。在废水处理厂废水中的应用

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摘要

A sensitive and highly selective enzyme-linked immunosorbent assay was developed for triclosan, one of the most common bactericides in personal care products, which is also considered as an emerging contaminant, given its presence in the effluents of sewage and wastewater treatment plants. From synthesized haptens, polyclonal rabbit antibodies against triclosan were raised. The best ELISA immunoassay was based on an antibody-coated format, yielding a detection limit of 0.03 μg L~(-1), an I_(50) of 3.85 μg L~(-1), and a dynamic range from 0.22 to 42.16 μg L~(-1), with little or no cross-reactivity (< 10%) to similarly structured compounds, including its metabolite methyltriclosan (CR < 6%). The assay was applied as a screening method to quantify triclosan in surface water and in wastewaters. After C18 solid-phase extraction, nanogram per liter concentrations were determined in effluents of urban wastewater treatment plants. The satisfactory recoveries achieved as well as the agreement between immunochemical and chromatographic methods (GC-MS) indicate its potential for either screening or laboratory quantification.
机译：针对个人护理产品中最常见的杀菌剂三氯生，开发了一种灵敏且高度选择性的酶联免疫吸附试验，由于三氯生存在于污水处理厂和污水处理厂的废水中，因此也被视为新兴污染物。从合成的半抗原中，产生了抗三氯生的多克隆兔抗体。最好的ELISA免疫分析是基于抗体包被的形式，检测限为0.03μgL〜（-1），I_（50）为3.85μgL〜（-1），动态范围为0.22至42.16微克L〜（-1），与类似结构的化合物，包括其代谢产物甲基三氯生（CR <6％），几乎没有或没有交叉反应性（<10％）。该测定法用作定量地表水和废水中三氯生的筛选方法。 C18固相萃取后，测定城市污水处理厂废水中的纳克每升浓度。所获得的令人满意的回收率以及免疫化学和色谱方法（GC-MS）之间的协议表明了其在筛选或实验室定量分析方面的潜力。

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