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首页> 外文期刊>Environmental Science & Technology >Physiological and Transcriptional Responses of Nitrosomonas europaea to Toluene and Benzene Inhibition
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Physiological and Transcriptional Responses of Nitrosomonas europaea to Toluene and Benzene Inhibition

机译：欧洲亚硝化单胞菌对甲苯和苯抑制的生理和转录响应

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摘要

Ammonia oxidizing bacteria (AOB) are inhibited by many compounds found in wastewater treatment plant (WWTP) influent including aromatic hydrocarbons. The detection of "sentinel genes" to identify the presence of aromatic hydrocarbons could be useful to WWTP operators. In this study, the transcriptomic responses of Nitrosomonas europaea during the cometabolism of benzene to phenol and toluene to benzyl alcohol and benzaldehyde were evaluated using whole genome Affymetrix microarrays and qRT-PCR. Benzyl alcohol and benzaldehyde were found notto inhibit N. europaea. However, phenol concentrations as low as 5 μM directly inhibited ammonia oxidation. Surprisingly, there were no significant up-or down-regulation of genes in N. europaea cells exposed to 20 μM toluene, which caused 50% inhibition of ammonia oxidation. Exposing N. europaea to 40 μM benzene, which caused a similar degree of inhibition, resulted in the up-regulation of seven adjacent genes, including NE 1545 (a putative pirin protein) and NE 1546 (a putative membrane protein), that appear to be involved with fatty-acid metabolism, lipid biosynthesis, and membrane protein synthesis. qRT-PCR analysis revealed that NE 1545 and NE 1546 were significantly up-regulated upon exposure to benzene and phenol, but not upon exposure to toluene. Transmission electron microscope images revealed a shift in outer cell structure in response to benzene exposure.
机译：氨氧化细菌（AOB）受废水处理厂（WWTP）进水中发现的许多化合物（包括芳烃）的抑制。 “前哨基因”的检测以识别芳香烃的存在可能对污水处理厂的经营者有用。在这项研究中，使用全基因组Affymetrix微阵列和qRT-PCR评估了欧洲亚硝化单胞菌在苯代谢为苯酚和甲苯代谢为苯甲醇和苯甲醛的过程中的转录组学反应。发现苯甲醇和苯甲醛不能抑制欧洲猪笼草。但是，低至5μM的苯酚浓度会直接抑制氨氧化。令人惊讶的是，在暴露于20μM甲苯的欧洲猪笼草细胞中，基因没有明显的上调或下调，这导致50％的氨氧化抑制。将欧洲猪笼草暴露于40μM苯中，产生相似程度的抑制作用，导致七个相邻基因上调，包括NE 1545（假定的pirin蛋白）和NE 1546（假定的膜蛋白），似乎与参与脂肪酸代谢，脂质生物合成和膜蛋白合成。 qRT-PCR分析表明，NE 1545和NE 1546在暴露于苯和苯酚时显着上调，但在暴露于甲苯时则未上调。透射电子显微镜图像显示响应于苯暴露，外部细胞结构发生了变化。

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  • 来源
    《Environmental Science & Technology》 |2008年第11期|p.4093-4098|共6页
  • 作者

    TYLER S. RADNIECKI; MARK E. DOLAN; LEWIS SEMPRINI;

  • 作者单位

    School of Chemical, Biological and Environmental Engineering;

    101 Gleeson Hall, Oregon State University,Corvallis, Oregon 97331;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 环境化学;
  • 关键词

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