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首页> 外文期刊>Environmental Science & Technology >Degradation of Lead-Contaminated Lignocellulosic Waste by Phanerochaete chrysosporium and the Reduction of Lead Toxicity
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Degradation of Lead-Contaminated Lignocellulosic Waste by Phanerochaete chrysosporium and the Reduction of Lead Toxicity

机译:Phanerochaete chrysosporium降解含铅木质纤维素废料并降低铅毒性

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摘要

Lead, as one of the most hazardous heavy metals to the environment interferes with lignocellulosic biomass bioconversion and carbon cycles in nature. The degradation of lead-polluted lignocellulosic waste and the restrain of lead hazards by solid-state fermentation with Phanerochaete chrysosporium were studied. Phanerochaete chrysosporium effectively degraded lignocellulose, formed humus and reduced active lead ions, even at the concentration of 400 mg/kg dry mass of lead. The highest lignocellulose degradation (56.8%) and organic matter loss (64.0%) were found at the concentration of 30 mg/kg of lead, and at low concentration of lead the capability of selective lignin biodegradation was enhanced. Microbial growth was delayed in polluted substrate at the initial stage of fermentation, and organic matter loss is correlated positively with microbial biomass after 12 day fermentation. It might be because Phanerochaete chrysosporium developed active defense mechanism to alleviate the lead toxicity. Scanning electron micrographs with energy spectra showed that lead was immobilized via two possible routes: adsorption and cation exchange on hypha, and the chelation by fungal metabolite. The present findings will improve the understandings about the degradation process and the lead immobilization pathway, which could be used as references for developing a fungi-based treatment technology for metal-contaminated lignocellulosic waste.
机译:铅作为对环境最有害的重金属之一,会干扰木质纤维素生物质的生物转化和自然界的碳循环。研究了Phanerochaete chrysosporium固态发酵对铅污染的木质纤维素废料的降解以及对铅危害的抑制作用。 Phanerochaete chrysosporium即使在铅干质量为400 mg / kg的情况下也能有效降解木质纤维素,形成腐殖质并减少活性铅离子。铅浓度为30 mg / kg时,木质纤维素降解最高(56.8%),有机质损失最高(64.0%),而铅浓度低时,选择性木质素的生物降解能力增强。在发酵初期,受污染的底物中微生物的生长被延迟,发酵12天后有机质的损失与微生物生物量呈正相关。可能是因为Phanerochaete chrysosporium开发了主动防御机制来减轻铅的毒性。扫描电子显微镜的能谱表明,铅通过两种可能的途径固定化:在菌丝上的吸附和阳离子交换,以及真菌代谢物的螯合。本研究结果将改善对降解过程和铅固定化途径的理解,可为开发基于真菌的金属污染木质纤维素废料处理技术提供参考。

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