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首页> 外文期刊>Environmental Science & Technology >Gene Expression Profiling in Daphnia magna PartⅠ: Concentration-Dependent Profiles Provide Support for the No Observed Transcriptional Effect Level
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Gene Expression Profiling in Daphnia magna PartⅠ: Concentration-Dependent Profiles Provide Support for the No Observed Transcriptional Effect Level

机译：大型蚤（Daphnia magna）中的基因表达谱分析（Ⅰ）：浓度依赖性谱为未观察到的转录作用水平提供了支持

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Ecotoxicogenomic approaches to environmental monitoring provide holistic information, offer insight into modes of action, and help to assess the causal agents and potential toxicity of effluents beyond the traditional end points of death and reproduction. Recent investigations of toxicant exposure indicate dose-dependent changes are a key issue in interpreting genomic studies. Additionally, there is interest in developing methods to integrate gene expression studies in environmental monitoring and regulation, and the No Observed Transcriptional Effect Level (NOTED has been proposed as a means for screening effluents and unknown chemicals for toxicity. However, computational methods to determine the NOTEL have yet to be established. Therefore, we examined effects on gene expression in Daphnia magna following exposure to Cu, Cd, and Zn over a range of concentrations including a tolerated, a sublethal, and a nearly acutely toxic concentration. Each concentration produced a distinct gene expression profile. We observed differential expression of a very few genes at tolerated concentrations that were distinct from the expression profiles observed at concentrations associated with toxicity. These results suggest that gene expression analysis may offer a strategy for distinguishing toxic and nontoxic concentrations of metals in the environment and provide support for a NOTEL for metal exposure in D. magna. Mechanistic insights could be inferred from the concentration-dependent gene expression profiles including metal specific effects on disparate metabolic processes such as digestion, immune response, development and reproduction, and less specific stress responses at higher concentrations.
机译：用于环境监测的生态毒物基因组学方法可提供整体信息，提供对作用方式的洞察力，并有助于评估超出传统死亡和繁殖终点的污水的病因和潜在毒性。最近对有毒物质暴露的研究表明，剂量依赖性变化是解释基因组研究的关键问题。此外，有兴趣开发将基因表达研究整合到环境监测和调节中的方法，并且已经提出了“无观察到的转录作用水平”（NOTED）作为筛选废水和未知化学品毒性的方法。 NOTEL尚无定论，因此，我们研究了在一定的浓度范围（包括可耐受的，亚致死的和几乎急性毒性的浓度）下暴露于Cu，Cd和Zn后，对水蚤对基因表达的影响。不同的基因表达谱：我们观察到极少数基因在耐受浓度下的差异表达，与在与毒性相关的浓度下观察到的表达谱不同，这些结果表明基因表达分析可以为区分金属的有毒和无毒浓度提供一种策略在环境中提供支持或NOTEL，用于D. magna中的金属暴露。从浓度依赖性基因表达谱可以推断出机理的见解，包括金属对不同代谢过程（如消化，免疫反应，发育和繁殖）的金属特异性作用，以及在较高浓度下特异性较低的应激反应。

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