...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental Science & Technology >Herbicidal Effects Of Sulfamethoxazole In Lemna Gibba: Using P-aminobenzoic Acid As A Biomarker Of Effect
【24h】

Herbicidal Effects Of Sulfamethoxazole In Lemna Gibba: Using P-aminobenzoic Acid As A Biomarker Of Effect

机译：磺胺甲基异恶唑在豆蔻果实中的除草作用：使用对氨基苯甲酸作为作用的生物标记

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Sulfamethoxazole (SMX) is among the most frequently detected antibiotics in the environment, heavily used in both human therapy and agriculture. Like other sulfonamides, SMX disrupts the folate biosynthetic pathway in bacteria, which was recently established as identical to that of plants, raising concerns over nontarget toxicity. Consequently, Lemna gibba was exposed to SMX to evaluate phytotoxic potency and mode of action (MOA) by HPLC-MS/MS measurement of p-aminobenzoic acid (pABA) metabolite levels, a precursor to folate biosynthesis and substrate of the target enzyme dihydropteroate synthase (DHPS). pABA levels were found to increase upon exposure to SMX following an exponential rise to a maxima regression model in a concentration-dependent manner. The EC_50 for pABA content was 3.36 μg/L, 20 times lower than that of fresh weight (61.6 μg/L) and 40 times lower than frond number (132 μg/L) responses. These results suggest that, as in bacteria, sulfonamide antibiotics specifically disrupt folate biosynthesis via inhibition of DHPS. Analysis of pABA concentrations appears to provide a sulfonamide-specific biomarker of effect based on MOA with exceptional diagnostic capacity and sensitivity compared to traditional morphological end points. Using the EC50 for pABA content, a potential hazard was identified for L. gibba exposed to SMX, which would not have been detected based upon traditional standardized morphological approaches.
机译：磺胺甲恶唑（SMX）是环境中最常检测到的抗生素之一，在人类治疗和农业中均大量使用。像其他磺酰胺一样，SMX破坏了细菌中的叶酸生物合成途径，该途径最近被证实与植物相同，从而引发了人们对非靶标毒性的担忧。因此，Lemna gibba暴露于SMX中，通过HPLC-MS / MS测量对氨基苯甲酸（pABA）代谢物，叶酸生物合成的前体和目标酶二氢蝶呤合酶的底物来评估植物毒性和作用方式（MOA）。 （DHPS）。发现在暴露于SMX后，pABA水平以浓度依赖的方式呈指数增长至最大值回归模型后增加。 pABA含量的EC_50为3.36μg/ L，比鲜重（61.6μg/ L）低20倍，比叶重（132μg/ L）低40倍。这些结果表明，与细菌一样，磺胺类抗生素通过抑制DHPS特异性破坏叶酸的生物合成。与传统形态学终点相比，基于MOA的pABA浓度分析似乎提供了磺酰胺特异性的生物标志物，具有卓越的诊断能力和灵敏度。使用EC50的pABA含量，可以确定暴露于SMX的g。L. gibba的潜在危害，根据传统的标准化形态学方法无法检测到。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号