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Estrogenic activity and steroid hormones in swine wastewater through a lagoon constructed-wetland system

机译:泻湖人工湿地系统在猪废水中的雌激素活性和类固醇激素

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摘要

Anaerobic lagoons and treatment wetlands are used worldwide to treat wastewater from dense livestock production facilities; however, there is very limited data on the hormonal activity of the wastewater effluent produced by these treatment systems. The objectives of this experiment were to measure (1) the hormonal activity of the initial effluent and (2) the effectiveness of a lagoon-constructed wetland treatment system for producing an effluent with a low hormonal activity. Wastewater samples were taken in April, July, and November 2004 and July 2005 from a lagoon-constructed wetland system at a swine farrowing facility. Estrogenic activity (in vitro E-screen assay), 17 beta-estradiol (E-2), and testosterone concentrations (LC/MS-MS) were measured. A high correlation was found between estradiol equivalents determined by E-screen and LC/MS-MS (R-2 = 0.82). Nutrient removal was measured to ensure that the wetlands were functioning in a manner similar to literature reports. Nutrient removals were typical for treatment wetlands: TKN 59-75% and orthophosphate 0-18%. Wetlands decreased estrogenic activity by 83-93%. Estrone was the most persistent estrogenic compound. Constructed wetlands produced effluents with estrogenic activity below the lowest equivalent E-2 concentration known to have an effect on fish (10 ng/L or similar to 37 x 10(-12) M).
机译:厌氧池和处理湿地在世界范围内用于处理密集的畜牧生产设施中的废水。然而,关于由这些处理系统产生的废水的激素活动的数据非常有限。该实验的目的是测量(1)初始废水的激素活性,以及​​(2)泻湖建造的湿地处理系统产生低激素活性废水的有效性。分别于2004年4月,7月,11月和2005年7月从猪场产卵设施的泻湖建造的湿地系统中采集了废水样品。测量了雌激素活性(体外E筛选试验),17种β-雌二醇(E-2)和睾丸激素浓度(LC / MS-MS)。通过电子筛查确定的雌二醇当量与LC / MS-MS之间存在高度相关性(R-2 = 0.82)。测量营养去除率以确保湿地以类似于文献报道的方式起作用。对于处理湿地,典型的营养去除是:TKN 59-75%和正磷酸盐0-18%。湿地使雌激素活性降低了83-93%。雌酮是最持久的雌激素化合物。人工湿地产生的废水中的雌激素活性低于已知对鱼类有影响的最低等效E-2浓度(10 ng / L或近似于37 x 10(-12)M)。

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