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首页> 外文期刊>Environmental Science & Technology >Analysis of Lagoon Samples from Different Concentrated Animal Feeding Operations for Estrogens and Estrogen Conjugates
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Analysis of Lagoon Samples from Different Concentrated Animal Feeding Operations for Estrogens and Estrogen Conjugates

机译：不同浓缩动物饲养操作中泻湖样品中雌激素和雌激素结合物的分析

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Although Concentrated Animal Feeding Operations (CAFOs) have been identified as potentially important sources for the release of estrogens into the environment, information is lacking on the concentrations of estrogens in whole lagoon effluents (including suspended solids) which are used for land application. Lagoons associated with swine, poultry, and cattle operations were sampled at three locations each for direct analysis for estrogens by GC/ MS/MS and estrogen conjugates by LC/MS/MS. Estrogen conjugates were also analyzed indirectly by first subjecting the same samples to enzyme hydrolysis. Solids from centrifuged samples were extracted for free estrogens to estimate total estrogen load. Total free estrogen levels (estrone, 17α-estradiol, 17β-estradiol, estriol) were generally higher in swine primary (1000-21000 ng/L), followed by poultry primary (1800-4000 ng/L), dairy secondary (370-550 ng/L), and beef secondary (22-24 ng/L) whole lagoon samples. Swine and poultry lagoons contained levels of 17α-estradiol comparable to those of 17β-estradiol. Confirmed estrogen conjugates included estrone-3-sulfate (2-91 ng/ L), 17β-estradiol-3-sulfate (8-44 ng/L), 17α-estradiol-3-sulfate (141-182 ng/L), and 17β-estradiol-17-sulfate (72-84 ng/L) in some lagoons. Enzymatic hydrolysis indicated the presence of additional unidentified estrogen conjugates not detected by the LC/MS/MS method. In most cases estrogen conjugates accounted for at least a third of the total estrogen equivalents. Collectively, these methods can be used to better determine estrogen loads from CAFO operations, and this research shows that estrogen conjugates contribute significantly to the overall estrogen load, even in different types of CAFO lagoons.
机译：尽管浓缩动物饲养业务（CAFO）已被确定为将雌激素释放到环境中的潜在重要来源，但仍缺乏用于土地应用的整个泻湖废水（包括悬浮固体）中雌激素浓度的信息。在三个位置分别采样与猪，家禽和牛操作相关的泻湖，分别通过GC / MS / MS分析雌激素和通过LC / MS / MS分析雌激素结合物。还通过首先对相同样品进行酶水解来间接分析雌激素结合物。提取离心样品中的固体中的游离雌激素，以估计总雌激素负荷。猪原发中的总游离雌激素水平（雌酮，17α-雌二醇，17β-雌二醇，雌三醇）通常较高（1000-21000 ng / L），其次是家禽（1800-4000 ng / L），乳制品（370- 550 ng / L）和次级（22-24 ng / L）牛肉整个泻湖样品。猪和家禽泻湖中的17α-雌二醇含量与17β-雌二醇相当。确认的雌激素结合物包括：雌二醇-3-硫酸盐（2-91 ng / L），17β-雌二醇-3-硫酸盐（8-44 ng / L），17α-雌二醇-3-硫酸盐（141-182 ng / L），泻湖中的17β-雌二醇-17-硫酸盐（72-84 ng / L）。酶促水解表明存在其他未鉴定的雌激素结合物，而LC / MS / MS方法未检测到。在大多数情况下，雌激素结合物至少占总雌激素当量的三分之一。总体而言，这些方法可用于更好地确定CAFO手术中的雌激素负荷，并且这项研究表明，即使在不同类型的CAFO泻湖中，雌激素结合物也对总体雌激素负荷做出了重大贡献。

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