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Phytochelatins and Antioxidant Systems Respond Differentially during Arsenite and Arsenate Stress in Hydrilla verticillata (L.f.) Royle

机译:轮叶藻(Hydilla verticillata(L.f.)Royle)的砷和砷酸盐胁迫期间,植物螯合物和抗氧化系统的反应不同

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Serious contamination of aquatic systems by arsenic (As) in different parts of the world calls for the development of an in situ cost-effective phytoremediation technology. In the present investigation, plants of Hydrilla verticillata (L.f.) Royle were exposed to various concentrations of arsenate (As~Ⅴ) (0-250 μM) and arsenite (As~Ⅲ) (0-25 μM) and analyzed for accumulation responses vis-a-vis biochemical changes. Total As accumulation was found to be higher in plants exposed to As~Ⅲ (315 μg g~(-1) dw at 25 μM) compared to As~Ⅴ (205 μg g~(-1) dw at 250 μM) after 7 d of treatment. Plants tolerated low concentrations of As~Ⅲ and As~Ⅴ by detoxifying the metalloid through augmented synthesis of thiols such as phytochelatins and through increased activity of antioxidant enzymes. While As~Ⅴ predominantly stimulated antioxidant enzyme activity, As~Ⅲ primarily caused enhanced levels of thiols. The maximum amount of As chelated by PCs was found to be about 39% in plants exposed to As~Ⅲ (at 10 μM) and 35% in As~Ⅴ-exposed plants (at 50 μM) after 4 d. Only the respective highest concentrations of As~Ⅲ (25 μM) and As~Ⅴ (250 μM) proved toxic for normal plant growth after prolonged treatment. Thus, H. verticillata forms a promising candidate for the phytoremediation of As contaminated water.
机译:在世界不同地区,砷对水生系统的严重污染要求开发一种原位经济有效的植物修复技术。在本次调查中,将藜芦(Lyd verticillata(Lf)Royle)的植物暴露于各种浓度的砷酸盐(As〜Ⅴ)(0-250μM)和砷酸盐(As〜Ⅲ)(0-25μM)中,并对其累积响应进行了分析。 -生化变化。发现暴露于As〜Ⅲ(25μM时为315μgg〜(-1)dw)的植物中的总As积累高于7〜7天后暴露于As〜Ⅴ(250μM时为205μgg〜(-1)dw)的植物。 d治疗。植物通过增加硫醇(例如植物螯合素)的合成以及增加抗氧化酶的活性来使准金属解毒,从而耐受低浓度的As〜Ⅲ和As〜Ⅴ。 As〜Ⅴ主要刺激抗氧化酶的活性,而As〜Ⅲ主要引起硫醇水平的提高。发现PC螯合的As的最大量在暴露于As〜Ⅲ的植物(10μM)中约为39%,在暴露于As〜Ⅴ的植物(50μM)中为35%。长时间处理后,只有最高浓度的As〜Ⅲ(25μM)和As〜Ⅴ(250μM)被证明对正常植物生长有毒。因此,H。verticillata成为对被As污染的水进行植物修复的有前途的候选者。

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