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首页> 外文期刊>Environmental Science & Technology >Sensitive Detection of Anaerobic Monochlorobenzene Degradation Using Stable Isotope Tracers
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Sensitive Detection of Anaerobic Monochlorobenzene Degradation Using Stable Isotope Tracers

机译：稳定同位素示踪剂灵敏检测厌氧一氯苯降解

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摘要

Microbial degradation of monochlorobenzene (MCB) under anaerobic conditions was investigated using a stable isotope tracer under in and ex situ conditions. In situ microcosms were incubated directly in an anoxic aquifer and carbon derived from [~(13)C_6]-MCB was found to be incorporated into the microbial biomass. In laboratory microcosms, amended with [~(13)C_6]-MCB, anaerobic mineralization of MCB was indicated by the production of ~(13)CO_2. Further, recovery of the ~(13)C-label in the fatty acids confirmed the assimilation of MCB-derived carbon into microbial biomass. The described approach may be applied to various other organic groundwater contaminants of concern using carbon (~(13)C) as well as other stable isotope tracers, such as nitrogen (~(15)N), allowing direct and sensitive detection of biodegradation.
机译：使用稳定的同位素示踪剂在原位和异位条件下研究了厌氧条件下一氯苯（MCB）的微生物降解。原位微观世界直接在缺氧含水层中温育，发现源自[〜（13）C_6] -MCB的碳被掺入微生物生物量中。在实验室缩影中，用[〜（13）C_6] -MCB修正，MCB的厌氧矿化由〜（13）CO_2的产生指示。此外，脂肪酸中〜（13）C-标记的回收证实了MCB衍生的碳同化为微生物生物质。所描述的方法可以应用于使用碳（〜（13）C）以及其他稳定的同位素示踪剂（例如氮（〜（15）N））引起的各种其他关注的有机地下水污染物，从而可以直接，灵敏地检测到生物降解。

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