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首页> 外文期刊>Environmental Science & Technology >Physiological Response to Persistent Organic Pollutants in Fish from Mountain Lakes: Analysis of Cyp1A Gene Expression in Natural Populations of Salmo trutta
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Physiological Response to Persistent Organic Pollutants in Fish from Mountain Lakes: Analysis of Cyp1A Gene Expression in Natural Populations of Salmo trutta

机译：高山湖泊鱼类对持久性有机污染物的生理响应：Salmo trutta自然种群中Cyp1A基因表达的分析

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摘要

Cytochrome p450 1A (CYP1A) gene expression in fish liver increases upon exposure to a variety of chemical compounds, including organochlorine compounds (OCs) and polycyclic aromatic hydrocarbons (PAHs). To use this physiological response as a marker of environmental impact, we developed and validated a set of primers to quantify CYP1A expression by qRT-PCR in the brown trout, Salmo trutta. These primers were used to explore the natural variability of CYP1A expression in 8 isolated populations (65 samples) from European remote lakes, in a geographical distribution encompassing the Tyrolean Alps, Pyrenees, Rila, Tatras, and Norwegian and Scottish mountains. CYP1A expression values varied more than 2 orders of magnitude among samples, with strong variations within each population. CYP1A expression values were significantly elevated in Tatras and Pyrenees fish populations, whereas the lowest median values were found in populations from the Tyrolean Alps and Rila. These values correlated with the content of different environmentally relevant pollutants in the sediments of the lakes harboring each fish population, particularly with HCB and 4,4'-DDE contents. To our knowledge, this works represents a first report of a physiological response linked to persistent organic pollutants in fish from mountain lakes.
机译：鱼肝中的细胞色素p450 1A（CYP1A）基因表达在暴露于多种化学化合物（包括有机氯化合物（OCs）和多环芳烃（PAHs））时会增加。为了将这种生理反应用作环境影响的标志，我们开发并验证了一套引物，用于通过qRT-PCR定量分析鳟鱼Salmo trutta中的CYP1A表达。这些引物用于探索欧洲偏远湖泊的8个分离种群（65个样品）中CYP1A表达的自然变异性，分布范围包括蒂罗尔阿尔卑斯山，比利牛斯山脉，里拉，塔特拉山和挪威和苏格兰山脉。样品中CYP1A表达值的变化幅度超过2个数量级，每个人群中的变化很大。 CYP1A表达值在Tatras和比利牛斯鱼群中显着升高，而在蒂罗尔阿尔卑斯山和里拉的群体中发现的中值最低。这些值与每个鱼类种群所在的湖泊沉积物中不同环境相关污染物的含量相关，尤其与六氯代苯和4,4'-DDE含量有关。就我们所知，这项工作代表了与高山湖泊鱼类中持久性有机污染物有关的生理反应的首次报道。

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