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首页> 外文期刊>Environmental Science & Technology >Role of Divalent Cations in Plasmid DNA Adsorption to Natural Organic Matter-Coated Silica Surface
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Role of Divalent Cations in Plasmid DNA Adsorption to Natural Organic Matter-Coated Silica Surface

机译：二价阳离子在质粒DNA吸附到天然有机物包覆的二氧化硅表面的作用

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摘要

The adsorption kinetics of supercoiled and linear plasmid DNA onto a natural organic matter (NOM)-coated silica surface are acquired using a quartz crystal microbalance with dissipation monitoring (QCM-D) in the presence of common divalent electrolytes CaCl_2 and MgCl_2. The adsorption kinetics of both DNA are noticeably higher in the presence of CaCl_2 compared to MgCl_2. We hypothesize that specific bridging between the DNA phosphate groups and NOM carboxyl functional groups in the presence of Ca~(2+) cations may lead to more efficient attachment than in the presence of Mg~(2+) cations, which are only likely to allow for charge neutralization. The influence of background Na~+ cations on the adsorption kinetics in the presence of CaCl_2 is found to be insignificant, while the presence of Na~+ leads to slower attachment kinetics in MgCl_2. Rinsing the DNA layer adsorbed in the presence of CaCl_2 with a solution of low NaCl concentration followed by deionized water does not result in observable detachment, indicating irreversibility of DNA adsorption. Instead, softening of the DNA layer adsorbed in the presence of CaCl_2 with background Na~+ occurs with the rinses due to the increase in electrostatic repulsion between the phosphate functional groups along the DNA backbone. In the case of the DNA layer adsorbed in the presence of CaCl_2 without background Na~+, softening of the layer does not occur with the rinses.
机译：在常见的二价电解质CaCl_2和MgCl_2的存在下，使用带有耗散监测的石英晶体微量天平（QCM-D），获得了超螺旋和线性质粒DNA在天然有机物（NOM）包覆的二氧化硅表面上的吸附动力学。与MgCl_2相比，在CaCl_2存在下，两种DNA的吸附动力学都明显更高。我们假设存在Ca〜（2+）阳离子时，DNA磷酸酯基团与NOM羧基官能团之间的特异性桥接可能比存在Mg〜（2+）阳离子时导致更有效的连接，后者仅可能允许电荷中和。发现在CaCl_2存在下背景Na〜+阳离子对吸附动力学的影响不明显，而Na〜+的存在导致MgCl_2中吸附动力学变慢。用低浓度的NaCl溶液，然后用去离子水冲洗在CaCl_2存在下吸附的DNA层，不能观察到分离，表明DNA吸附不可逆。取而代之的是，由于沿DNA骨架的磷酸酯官能团之间的静电排斥力的增加，在漂洗过程中，在CaCl_2存在下被背景Na〜+吸附的DNA层发生软化。对于在没有背景Na +的情况下在存在CaCl 2的情况下吸附的DNA层的情况，用冲洗液不会使该层变软。

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  • 来源
    《Environmental Science & Technology》 |2007年第15期|5370-5375|共6页
  • 作者

    THANH H. NGUYEN; KAI LOON CHEN;

  • 作者单位

    Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, 3230 Newmark Laboratory, Urbana, Illinois 61801;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 环境化学;
  • 关键词

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