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首页> 外文期刊>Environmental Science & Technology >Gibberellic Acid, Kinetin, and the Mixture Indole—3-Acetic Acid—Kinetin Assisted with EDTA-Induced Lead Hyperaccumulation in Alfalfa Plants
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Gibberellic Acid, Kinetin, and the Mixture Indole—3-Acetic Acid—Kinetin Assisted with EDTA-Induced Lead Hyperaccumulation in Alfalfa Plants

机译：苜蓿植物中的赤霉素，激动素和吲哚3-乙酸-激动素的混合物在EDTA诱导下导致铅超积累

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摘要

There are a few plant species considered potential hyperac-cumulators for heavy metals, particularly lead (Pb). In this study, alfalfa plants grown in hydroponics were exposed to Pb at 40 mg/L, ethylenediaminetetraacetic acid (EDTA) equimolar to Pb, and 1,10, and 100 μM concentrations of the phytohormones indole-3-acetic acid (IAA), gibberellic acid (GA), and kinetin (KN) and a mixture of IAA and KN at 100 μM each. Metal quantification by inductively coupled plasma/optical emission spectroscopy demonstrated that plants treated with Pb/EDTA plus KN at 1,10, and 100 μM increased the Pb concentration in alfalfa leaves (compared to Pb alone) by factors of 17, 43, and 67, respectively, and by factors of 2, 5, and 8, respectively, compared to the Pb/EDTA treatment. The correlation coefficient between the Pb concentration in leaves and the concentrations of KN in the medium was 0.9993. In addition, the leaves of plants exposed to a Pb/EDTA/100 μM IAA-KN mixture had approximately 9500 mg of Pb/kg of dry weight, demonstrating that non-Pb hyperaccumulating plants could hyperaccumulate Pb when treated with EDTA and a mixture of IAA-KN. The X-ray absorption spectroscopic studies demonstrated that the absorption and translocation of Pb was in the same oxidation state as the supplied Pb(II).
机译：有一些植物物种被认为是潜在的重金属，特别是铅（Pb）的超高累积量。在这项研究中，将以水培法生长的苜蓿植物暴露于40 mg / L的Pb，与Pb等摩尔的乙二胺四乙酸（EDTA）以及浓度为1,10和100μM的植物激素吲哚-3-乙酸（IAA），赤霉素（GA）和激动素（KN）以及分别为100μM的IAA和KN的混合物。通过电感耦合等离子体/光发射光谱法对金属进行的定量分析表明，用1,10和100μM的Pb / EDTA加KN处理的植物使苜蓿叶中的Pb浓度（与单独的Pb相比）增加了17、43和67倍。与Pb / EDTA处理相比，分别减少了2倍，5倍和8倍。叶片中铅的浓度与培养基中KN的相关系数为0.9993。此外，暴露于Pb / EDTA / 100μMIAA-KN混合物的植物叶片具有约9500 mg Pb / kg干重，这表明非PB超富集植物在用EDTA和IAA-KN。 X射线吸收光谱研究表明，Pb的吸收和易位与所提供的Pb（II）处于相同的氧化态。

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