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首页> 外文期刊>Environmental Science & Technology >Toxicogenomic Analysis Provides New Insights into Molecular Mechanisms of the Sublethal Toxicity of 2,4,6-Trinitrotoluene in Eisenia fetida
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Toxicogenomic Analysis Provides New Insights into Molecular Mechanisms of the Sublethal Toxicity of 2,4,6-Trinitrotoluene in Eisenia fetida

机译：毒理基因组学分析提供了新的见解，在2,4,6-三硝基甲苯在致命的埃塞俄比亚毒性的分子机制。

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摘要

Xenobiotics such as explosives and pesticides released into the environment can have lethal and sublethal impacts on soil organisms such as earthworms with potential subsequent impacts at highertrophic levels. To better understand the molecular toxicological mechanisms of 2,4,6-trinitrotoluene (TNT), a commonly used explosive, in Eisenia fetida, earthworms were exposed to a gradient of TNT-spiked soils for 28 days and impacts on gene expression were examined using a 4032 cDNA microarray. Reproduction was increased at low doses of TNT, whereas high doses of TNT reduced juvenile production. On the basis of reproduction responses to TNT, four treatments, that is, control, 2,10.6, and 38.7 mg/kg, were selected for gene expression studies in a balanced interwoven loop design microarray experiment in which the expression of 311 transcripts was significantly affected. Reverse-transcription quantitative polymerase chain reaction (RT-QPCR) data on 68 selected differentially and nondifferentially expressed transcripts showed a significant correlation with microarray results. The expression of genes involved in multiple biological processes was altered, including muscle contraction, neuronal signaling and growth, ubiquitinylation, fibrinolysis and coagulation, iron and calcium homeostasis, oxygen transport, and immunity. Chitinase activity assays confirmed down-regulation of chitinase genes as indicated by array and RT-QPCR data. An acute toxicity test provided evidence that dermal contact with TNT can cause bleeding, inflammation, and constriction, which may be explained by gene expression results. Sublethal doses of TNT affected the nervous system, caused blood disorders similar to methemoglobinemia, and weakened immunity in E. fetida.
机译：释放到环境中的诸如炸药和农药之类的异生物可能对土壤生物（如worm）产生致命和亚致死影响，并在较高营养水平下可能产生后续影响。为了更好地了解2,4,6-三硝基甲苯（TNT）（一种常见的炸药）的分子毒理学机理，在Eisenia fetida中，将were暴露于TNT加标的土壤中28天，然后使用4032 cDNA微阵列。低剂量的TNT会增加生殖，而高剂量的TNT会减少少年的生产。根据对TNT的繁殖反应，在平衡的交织环设计微阵列实验中选择了四种处理方法，即对照，2,10.6和38.7 mg / kg进行基因表达研究，其中311转录本的表达明显受到影响。逆转录定量聚合酶链反应（RT-QPCR）数据在68个差异和非差异表达的转录本上显示出与微阵列结果显着相关。涉及多个生物学过程的基因表达发生了改变，包括肌肉收缩，神经元信号传导和生长，泛素化，纤维蛋白溶解和凝结，铁和钙稳态，氧转运和免疫力。几丁质酶活性测定证实了几丁质酶基因的下调，如阵列和RT-QPCR数据所示。一项急性毒性试验提供了证据，表明真皮与TNT接触可引起出血，炎症和收缩，这可由基因表达结果解释。亚致死剂量的TNT会影响神经系统，引起类似于高铁血红蛋白血症的血液疾病，并且会削弱Fetida的免疫力。

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