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首页> 外文期刊>Environmental Science & Technology >Replicability of bacterial communities in denitrifying bioreactors as measured by PCR/T-RFLP analysis
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Replicability of bacterial communities in denitrifying bioreactors as measured by PCR/T-RFLP analysis

机译：通过PCR / T-RFLP分析测得的反硝化生物反应器中细菌群落的可复制性

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Bioreactors hold great promise for treating graywater in an advanced life support system for space applications. However, questions remain regarding the reproducibility and reliability of biological systems for long-term use. Although there have been numerous studies on ground-based biological systems, most studies focus on a single reactor or a simple (single carbon) waste stream. There have been very few studies on microbial communities in replicate reactors using a nonsterile, complex waste stream. In this report, we describe the characterization of five replicate denitrifying reactors receiving a complex feed, including urine and limb washes from donors at Johnson Space Center over a 100-day period. Denitrifying conditions were employed because of the ease in adding a terminal electron acceptor to the bioreactor. Bacterial populations were tracked by 16S rRNA and nosZ genes T-RFLP analysis to target the total and denitrifying microbial communities. The results demonstrated reproducible biological communities with nearly identical performance that slowly changed with time and exhibited low variability with respect to the bacterial community (T-RFLP peak area) in all reactors. These results suggest that, when designed for replication, bioreactors are not stochastic systems exhibiting chaotic behavior, but are biological systems that can be highly reproducible and reliable.
机译：生物反应器在用于太空应用的先进生命支持系统中处理灰水具有广阔的前景。但是，对于长期使用的生物系统的可再现性和可靠性仍然存在疑问。尽管对地面生物系统进行了大量研究，但大多数研究集中在单个反应器或简单（单碳）废物流上。关于使用非无菌，复杂废物流的复制反应器中微生物群落的研究很少。在本报告中，我们描述了五个复杂的反硝化反应器的特征，这些反应器接收了复杂的进料，包括在约翰逊航天中心在100天的时间内从捐助者那里得到的尿液和肢体清洗液。使用反硝化条件是因为容易向生物反应器中添加末端电子受体。通过16S rRNA和nosZ基因T-RFLP分析来跟踪细菌种群，以靶向总的和反硝化微生物群落。结果表明，在所有反应堆中，可再现的生物群落具有几乎相同的性能，其性能随时间缓慢变化，并且相对于细菌群落表现出低变异性（T-RFLP峰面积）。这些结果表明，当设计用于复制时，生物反应器不是表现出混沌行为的随机系统，而是可以高度重现和可靠的生物系统。

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