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首页> 外文期刊>Environmental Science & Technology >Plasma proteome analysis reveals the geographical origin and liver tumor status of dab (Limanda limanda) from UK marine waters
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Plasma proteome analysis reveals the geographical origin and liver tumor status of dab (Limanda limanda) from UK marine waters

机译：血浆蛋白质组学分析揭示了英国海水中DAB（Limanda limanda）的地理起源和肝肿瘤状况

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The flatfish species dab (Limanda limanda) is the sentinel for offshore marine monitoring in the United Kingdom National Marine Monitoring Programme (NMMP). At certain sites in the North and Irish Seas, the prevalence of macroscopic liver tumors can exceed 10%. The plasma proteome of these fish potentially contains reporter proteins or "biomarkers" that may enable development of diagnostic tests for liver cancer and further our understanding of the disease. Following selection of sample groups by quality-assured histopathology ("phenotype anchoring"), we used surface-enhanced laser desorption/ionization (SELDI) time-of-flight mass spectrometry to produce proteomic profiles of plasma from 213 dab collected during the 2004 UK NMMP. The resulting protein profiles were compared between fish from the North and Irish Seas and between fish with liver neoplasia or nondiseased liver. Significant differences were found between the plasma proteomes of dab from the North Sea and Irish Sea, which in conjunction with artificial neural networks can correctly determine from which sea dab were captured in 85% of the cases. In addition, the presence of liver tumors is associated with significant changes in the plasma proteome. We conclude that SELDI-based plasma profiling is potentially of use in nonlethal marine monitoring using wild sentinels such as dab. Furthermore, accurate selection of sample groups is critical for avoiding effects of confounding factors such as age, gender, and geographic origin of samples.
机译：在英国国家海洋监测计划（NMMP）中，比目鱼品种dab（Limanda limanda）是近海海洋监测的定点。在北海和爱尔兰海的某些地点，肉眼可见的肝肿瘤的发生率可超过10％。这些鱼的血浆蛋白质组中可能含有报告蛋白或“生物标志物”，它们可能有助于肝癌诊断测试的发展，并进一步加深我们对该病的了解。在通过质量保证的组织病理学（“表型锚定”）选择样本组之后，我们使用表面增强的激光解吸/电离（SELDI）飞行时间质谱仪从2004年英国期间收集的213 dab产生血浆的蛋白质组学概况NMMP。比较了来自北海和爱尔兰海的鱼类以及患有肝肿瘤或未患病肝脏的鱼类之间的蛋白质分布。在北海和爱尔兰海的dab血浆蛋白质组之间发现了显着差异，结合人工神经网络可以正确确定在85％的情况下是从哪个海dab中捕获的。另外，肝肿瘤的存在与血浆蛋白质组的显着变化有关。我们得出的结论是，基于SELDI的血浆谱分析可能会用于使用野生标记（例如dab）进行的非致命性海洋监测中。此外，准确选择样本组对于避免混淆因素（例如年龄，性别和样本的地理来源）的影响至关重要。

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